Microbiology & Fermentation Technology Note
Distribution of Methanotrophs in the Phyllosphere
2012 Volume 76 Issue 8 Pages 1580-1583
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2012 Volume 76 Issue 8 Pages 1580-1583
Plants have been reported to emit methane as well as methanol originating in their cell-wall constituents. We investigated methanotrophs in the phyllosphere by the enrichment culture method with methane as sole carbon source. We enriched methanotrophs from the leaves, flowers, bark, and roots of various plants. Analysis of the pmoA and mxaF genes retrieved from the enrichment cultures revealed that methanotrophs closely related to the genera Methylomonas, Methylosinus, and Methylocystis inhabit not only the rhizosphere but also the phyllosphere, together with methanol-utilizing bacteria.
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