Effects of Depletion of Glutathione on Abscisic Acid- and Methyl Jasmonate-Induced Stomatal Closure in Arabidopsis thaliana

Nasima AKTER; Muhammad Abdus SOBAHAN; Misugi URAJI; Wenxiu YE; Mohammad Anowar HOSSAIN; Izumi C. MORI; Yoshimasa NAKAMURA; Yoshiyuki MURATA

doi:10.1271/bbb.120384

Biochemistry & Molecular Biology Regular Papers

Effects of Depletion of Glutathione on Abscisic Acid- and Methyl Jasmonate-Induced Stomatal Closure in Arabidopsis thaliana

Nasima AKTER, Muhammad Abdus SOBAHAN, Misugi URAJI, Wenxiu YE, Mohammad Anowar HOSSAIN, Izumi C. MORI, Yoshimasa NAKAMURA, Yoshiyuki MURATA

Author information

Nasima AKTER
Division of Bioscience, Graduate School of Natural Science and Technology, Okayama University
Muhammad Abdus SOBAHAN
Division of Bioscience, Graduate School of Natural Science and Technology, Okayama University
Misugi URAJI
Division of Bioscience, Graduate School of Natural Science and Technology, Okayama University
Wenxiu YE
Division of Bioscience, Graduate School of Natural Science and Technology, Okayama University
Mohammad Anowar HOSSAIN
Division of Bioscience, Graduate School of Natural Science and Technology, Okayama University
Izumi C. MORI
Institute of Plant Science and Resources (IPSR), Okayama University
Yoshimasa NAKAMURA
Division of Bioscience, Graduate School of Natural Science and Technology, Okayama University
Yoshiyuki MURATA
Division of Bioscience, Graduate School of Natural Science and Technology, Okayama University

Keywords: abscisic acid, cytosolic alkalization, glutathione, methyl jasmonate, reactive oxygen species

JOURNAL FREE ACCESS

2012 Volume 76 Issue 11 Pages 2032-2037

DOI https://doi.org/10.1271/bbb.120384

View "Advance Publication" version (November 7, 2012).

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Abstract

Glutathione (GSH) is involved in abscisic acid (ABA)- and methyl jasmonate (MeJA)-induced stomatal closure in Arabidopsis thaliana. In this study, we examined the effects of GSH-decreasing chemicals, p-nitrobenzyl chloride (PNBC), iodomethane (IDM), and ethacrynic acid (EA), on ABA- and MeJA-induced stomatal closure in Arabidopsis. Treatments with PNBC, IDM, and EA decreased GSH contents in guard cells. Depletion of GSH by PNBC and IDM enhanced ABA- and MeJA-induced stomatal closure and inhibition of light-induced stomatal opening by ABA, whereas EA did not enhance either ABA- and MeJA-induced stomatal closure or inhibition of light-induced stomatal opening by ABA. Depletion of GSH did not significantly increase the production of the reactive oxygen species (ROS), cytosolic alkalization, or cytosolic Ca²⁺ oscillation induced by ABA and MeJA. These results indicate that depletion of GSH enhances ABA- and MeJA-induced stomatal closure without affecting ROS production, cytosolic alkalization, or cytosolic Ca²⁺ oscillation in guard cells of Arabidopsis.

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