Bioscience, Biotechnology, and Biochemistry
Online ISSN : 1347-6947
Print ISSN : 0916-8451
Biochemistry & Molecular Biology Notes
A Distinct Binding Mode of Archaeal Ribonuclease P Proteins to RNA
Masato ISHIHARAEtsuko NISHIMOTOShoji YAMASHITAYoshimitsu KAKUTAMakoto KIMURA
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JOURNAL FREE ACCESS

2012 Volume 76 Issue 12 Pages 2335-2337

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Abstract

The ribonuclease P (RNase P) in the hyperthermophilic archaeon Pyrococcus horikoshii comprises RNA (PhopRNA) and five proteins. We analyzed the RNA binding mode of the protein, using a pair of complementary fluorescence-labeled oligoribonucleotides. Fluorescence resonance energy transfer (FRET)-based assays suggested that the RNase P proteins assist PhopRNA in attaining a functionally active conformation via a distinct mode of binding.

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© 2012 by Japan Society for Bioscience, Biotechnology, and Agrochemistry
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