Toxoflavin Lyase Enzyme as a Marker for Selecting Potato Plant Transformants

Mi-Sun KIM; HyunSoon KIM; Jae Sun MOON; Ingyu HWANG; Hyouk JOUNG; Jae-Heung JEON

doi:10.1271/bbb.120593

Biochemistry & Molecular Biology Notes

Toxoflavin Lyase Enzyme as a Marker for Selecting Potato Plant Transformants

Mi-Sun KIM, HyunSoon KIM, Jae Sun MOON, Ingyu HWANG, Hyouk JOUNG, Jae-Heung JEON

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Keywords: toxoflavin lyase (tflA), selection marker system, light-dependence, transformation efficiency

JOURNAL FREE ACCESS

2012 Volume 76 Issue 12 Pages 2354-2356

DOI https://doi.org/10.1271/bbb.120593

View "Advance Publication" version (December 7, 2012).

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Abstract

This study established a new system for potato transformation using toxoflavin as selection agent and toxoflavin lyase (tflA) as selectable marker gene. Potato plants expressing tflA was successfully transformed on toxoflavin medium with 27% efficiency, similar to that for the hygromycin/hpt selection system. The transgenic potato expressing tflA also showed resistance to Burkholderia glumea infection.

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