Expression and Characterization of a Glutamate Decarboxylase from Lactobacillus brevis 877G Producing γ-Aminobutyric Acid

Myung-Ji SEO; Young-Do NAM; So-Young LEE; So-Lim PARK; Sung-Hun YI; Seong-Il LIM

doi:10.1271/bbb.120785

Food & Nutrition Science Notes

Expression and Characterization of a Glutamate Decarboxylase from Lactobacillus brevis 877G Producing γ-Aminobutyric Acid

Myung-Ji SEO, Young-Do NAM, So-Young LEE, So-Lim PARK, Sung-Hun YI, Seong-Il LIM

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Keywords: γ-aminobutyric acid, characterization, expression, glutamate decarboxylase, Lactobacillus brevis

JOURNAL FREE ACCESS

2013 Volume 77 Issue 4 Pages 853-856

DOI https://doi.org/10.1271/bbb.120785

View "Advance Publication" version (April 7, 2013).

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Abstract

The glutamate decarboxylase of γ-aminobutyric acid-producing Lactobacillus brevis 877G (LbGAD) was expressed in Escherichia coli. The optimal pH and temperature for the purified LbGAD activity were respectively determined to be pH 5.2 and 45 °C. CaCl₂ was shown to be a potent activator of this LbGAD activity. The kinetic parameters for LbGAD were a K_m value of 3.6 mmol/L and a V_max value of 0.06 mmol/L/min for L-monosodium glutamate.

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