Bioscience, Biotechnology, and Biochemistry
Online ISSN : 1347-6947
Print ISSN : 0916-8451
Microbiology & Fermentation Technology Regular Papers
Enzymatic Production of L-Alanyl-L-glutamine by Recombinant E. coli Expressing α-Amino Acid Ester Acyltransferase from Sphingobacterium siyangensis
Yoshinori HIRAOYasuhiro MIHARAIkuo KIRAIsao ABEKenzo YOKOZEKI
Author information
JOURNAL FREE ACCESS

2013 Volume 77 Issue 3 Pages 618-623

Details
Abstract

An enzymatic production method for synthesizing L-alanyl-L-glutamine (Ala-Gln) from L-alanine methyl ester hydrochloride (AlaOMe) and L-glutamine (Gln) was developed in this study. The cultivation conditions for an Escherichia coli strain overexpressing α-amino acid ester acyltransferase from Sphingobacterium siyangensis AJ 2458 (SAET) and reaction conditions for Ala-Gln production were optimized. A high cell density culture broth prepared by fed-batch cultivation showed 440 units/mL of Ala-Gln-producing activity. In addition, an Ala-Gln-producing reaction using intact E. coli cells overexpressing SAET under optimum conditions was conducted. A total Ala-Gln yield of 69.7 g/L was produced in 40 min. The molar yield was 67% against both AlaOMe and Gln.

Content from these authors

This article cannot obtain the latest cited-by information.

© 2013 by Japan Society for Bioscience, Biotechnology, and Agrochemistry
Previous article Next article
feedback
Top