Determination by LC-MS of Juvenile Hormone Titers in Hemolymph of the Silkworm, Bombyx mori

Kenjiro FURUTA; Akio ICHIKAWA; Mika MURATA; Eiichi KUWANO; Tetsuro SHINODA; Takahiro SHIOTSUKI

doi:10.1271/bbb.120883

Analytical Chemistry Regular Paper

Determination by LC-MS of Juvenile Hormone Titers in Hemolymph of the Silkworm, Bombyx mori

Kenjiro FURUTA, Akio ICHIKAWA, Mika MURATA, Eiichi KUWANO, Tetsuro SHINODA, Takahiro SHIOTSUKI

Author information

Keywords: juvenile hormone, titration, silkworm, Bombyx mori, liquid chromatography-mass spectrometry (LC-MS)

JOURNAL FREE ACCESS

2013 Volume 77 Issue 5 Pages 988-991

DOI https://doi.org/10.1271/bbb.120883

View "Advance Publication" version (May 7, 2013).

Details

Abstract

Juvenile hormone (JH) I, II and III in the hemolymph of the silkworm, Bombyx mori were quantified by liquid chromatography-mass spectrometry (LC-MS). JHs were treated with methanol and trifluoroacetic acid to convert into JH methoxyhydrines (JH-MHs). The key to the analytical condition for JH-MHs was the addition of 5 µM sodium acetate to the eluting solution. Each JH-MH was observed as the sodium adduct ion with good sensitivity. This improved method enabled the titration of JH I, II and III in hemolymph of the silkworm to be monitored from the 3rd instar through to the early pupal stage. A peak of JH I was observed immediately after ecdysis in the 3rd and 4th instar stages. The JH I titer sharply decreased on day 1 and reached the lowest level before ecdysis, but there was no peak at the beginning of the 5th stadium, and no apparent increase was observed until pupation.

Corresponding author

Register with J-STAGE for free!