Differences in the Roles of a Glutamine Amidotransferase Subunit of Pyridoxal 5'-Phosphate Synthase between Bacillus circulans and Bacillus subtilis

Shiori ITAGAKI; Minami HAGA; Yuji OIKAWA; Ayaka SAKODA; Yoshie OHKE; Hiroshi SAWADA; Tadashi EGUCHI; Hideyuki TAMEGAI

doi:10.1271/bbb.130132

Biochemistry & Molecular Biology Regular Papers

Differences in the Roles of a Glutamine Amidotransferase Subunit of Pyridoxal 5'-Phosphate Synthase between Bacillus circulans and Bacillus subtilis

Shiori ITAGAKI, Minami HAGA, Yuji OIKAWA, Ayaka SAKODA, Yoshie OHKE, Hiroshi SAWADA, Tadashi EGUCHI, Hideyuki TAMEGAI

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Shiori ITAGAKI
Department of Correlative Study of Physics and Chemistry, Graduate School of Integrated Basic Sciences, Nihon University
Minami HAGA
Department of Correlative Study of Physics and Chemistry, Graduate School of Integrated Basic Sciences, Nihon University
Yuji OIKAWA
Department of Correlative Study of Physics and Chemistry, Graduate School of Integrated Basic Sciences, Nihon University
Ayaka SAKODA
Department of Correlative Study of Physics and Chemistry, Graduate School of Integrated Basic Sciences, Nihon University
Yoshie OHKE
Department of Chemistry, College of Humanities and Sciences, Nihon University
Hiroshi SAWADA
Department of Correlative Study of Physics and Chemistry, Graduate School of Integrated Basic Sciences, Nihon University
Department of General Studies, College of Humanities and Sciences, Nihon University
Tadashi EGUCHI
Department of Chemistry and Materials Science, Graduate School of Science and Engineering, Tokyo Institute of Technology
Hideyuki TAMEGAI
Department of Correlative Study of Physics and Chemistry, Graduate School of Integrated Basic Sciences, Nihon University
Department of Chemistry, College of Humanities and Sciences, Nihon University

Keywords: 2-deoxy-scyllo-inosose synthase, BtrC2, dual functions, pyridoxal 5'-phosphate biosynthesis, enzyme stabilization

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2013 Volume 77 Issue 7 Pages 1481-1485

DOI https://doi.org/10.1271/bbb.130132

View "Advance Publication" version (July 7, 2013).

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Abstract

BtrC2 of the butirosin producer Bacillus circulans is a non-catalytic subunit of 2-deoxy-scyllo-inosose (DOI) synthase that is involved in butirosin biosynthesis, and also a homolog of glutamine amidotransferase subunit (PdxT) of pyridoxal 5'-phosphate (PLP) synthase of Bacillus subtilis. BtrC2 has been found to have functions in B. circulans both in primary and secondary metabolism. In this study, we investigated the properties of PdxT of B. subtilis in order to determine whether the property of enzyme stabilization is universal among PdxT homologs. Complementation with PdxT in the btrC2 disruptant of B. circulans restored the growth and short-term production of antibiotics, but long-term production of antibiotics cannot be restored. Additionally, PdxT did not bind physically with or stabilize BtrC. Our results indicate that the function of BtrC2 in secondary metabolism is specific properties, not universal among PdxT homologs.

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