Long-Chain Free Fatty Acid Profiling Analysis by Liquid Chromatography–Mass Spectrometry in Mouse Treated with Peroxisome Proliferator-Activated Receptor α Agonist

Haruya TAKAHASHI; Hideyuki SUZUKI; Kunihiro SUDA; Yota YAMAZAKI; Akihiro TAKINO; Young-Il KIM; Tsuyoshi GOTO; Yoko IIJIMA; Koh AOKI; Daisuke SHIBATA; Nobuyuki TAKAHASHI; Teruo KAWADA

doi:10.1271/bbb.130572

Food & Nutrition Science Regular Papers

Long-Chain Free Fatty Acid Profiling Analysis by Liquid Chromatography–Mass Spectrometry in Mouse Treated with Peroxisome Proliferator-Activated Receptor α Agonist

Haruya TAKAHASHI, Hideyuki SUZUKI, Kunihiro SUDA, Yota YAMAZAKI, Akihiro TAKINO, Young-Il KIM, Tsuyoshi GOTO, Yoko IIJIMA, Koh AOKI, Daisuke SHIBATA, Nobuyuki TAKAHASHI, Teruo KAWADA

Author information

Haruya TAKAHASHI
Laboratory of Molecular Functions of Food, Division of Food Science and Biotechnology, Graduate School of Agriculture, Kyoto University
Hideyuki SUZUKI
Kazusa DNA Research Institutes
Kunihiro SUDA
Kazusa DNA Research Institutes
Yota YAMAZAKI
Laboratory of Molecular Functions of Food, Division of Food Science and Biotechnology, Graduate School of Agriculture, Kyoto University
Akihiro TAKINO
Laboratory of Molecular Functions of Food, Division of Food Science and Biotechnology, Graduate School of Agriculture, Kyoto University
Young-Il KIM
Laboratory of Molecular Functions of Food, Division of Food Science and Biotechnology, Graduate School of Agriculture, Kyoto University
Tsuyoshi GOTO
Laboratory of Molecular Functions of Food, Division of Food Science and Biotechnology, Graduate School of Agriculture, Kyoto University
Research Unit for Physiological Chemistry, Kyoto University
Yoko IIJIMA
Kazusa DNA Research Institutes
Koh AOKI
Kazusa DNA Research Institutes
Daisuke SHIBATA
Kazusa DNA Research Institutes
Nobuyuki TAKAHASHI
Laboratory of Molecular Functions of Food, Division of Food Science and Biotechnology, Graduate School of Agriculture, Kyoto University
Research Unit for Physiological Chemistry, Kyoto University
Teruo KAWADA
Laboratory of Molecular Functions of Food, Division of Food Science and Biotechnology, Graduate School of Agriculture, Kyoto University
Research Unit for Physiological Chemistry, Kyoto University

Keywords: peroxisome proliferator-activated receptor α (PPARα), free fatty acid (FFA), ultra-high-performance liquid chromatography-mass spectrometry (UPLC-MS)

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Supplementary material

2013 Volume 77 Issue 11 Pages 2288-2293

DOI https://doi.org/10.1271/bbb.130572

View "Advance Publication" version (November 7, 2013).

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Abstract

A change in the free fatty acid (FFA) profile reflects an alteration in the lipid metabolism of peripheral tissue. A high-throughput quantitative analysis method for individual FFAs therefore needs to be established. We report here an optimized LC-MS assay for a high-throughput and high-sensitivity analysis of the 10 major long-chain FFAs in mouse plasma and liver. This assay enables quantification of individual FFAs by using trace amounts of samples (2 µL of plasma and 10 mg of liver tissue). We apply this method to analyze the FFA profile of plasma and liver samples from an obese mouse model treated with bezafibrate, the peroxisome proliferator-activated receptor α (PPARα) agonist, and show a change in the FFA profile, particularly in the palmitoleic and oleic acid contents. This assay is useful for quantifying individual FFAs and helpful for monitoring the condition of lipid metabolism.

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