Asymmetrical diadenosine 5′,5″-P¹,P⁴-tetraphosphate hydrolase (EC 3.6.1.17) from human malaria parasite Plasmodium falciparum was expressed in Escherichia coli, purified to homogeneity, and characterized for the first time as a biological target for chemotherapeutic agents against malaria. Plasmodium falciparum Ap₄A (PfAp₄A) hydrolase not only catalyzes diadenosine 5′,5″-P¹,P⁴-tetraphosphate (Ap₄A) to ATP and AMP, but also diadenosine 5′,5″-P¹,P⁵-pentaphosphate (Ap₅A) to ATP and ADP. Marked enzyme heat stability corresponding to the highest level of activity was observed at 60°C. The recombinant enzyme showed maximal activity in the presence of 5 mM Mg²⁺ ions. Kinetic analysis revealed the values of K_m and K_cat as 0.6 µM and 2.5 min⁻¹, respectively. Comparative protein modeling indicated an additional space in the substrate binding site of the parasitic enzyme compared with that of humans. Mutagenic analysis of the amino acid residue (Pro133) forming the additional space revealed a 5-fold increase in the wild-type K_m value when replaced by a smaller (Ala) residue. Furthermore, catalytic activity was markedly affected by introducing a larger residue (Phe), thus creating the potential to develop a specific inhibitor of PfAp₄A hydrolase.