Extracorporeal Low-Energy Shock-Wave Therapy Exerts Anti-Inflammatory Effects in a Rat Model of Acute Myocardial Infarction

Yuzuru Abe; Kenta Ito; Kiyotaka Hao; Tomohiko Shindo; Tsuyoshi Ogata; Yuta Kagaya; Ryo Kurosawa; Kensuke Nishimiya; Kimio Satoh; Satoshi Miyata; Kazuyoshi Kawakami; Hiroaki Shimokawa

doi:10.1253/circj.CJ-14-0230

Ischemic Heart Disease

Extracorporeal Low-Energy Shock-Wave Therapy Exerts Anti-Inflammatory Effects in a Rat Model of Acute Myocardial Infarction

Yuzuru Abe, Kenta Ito, Kiyotaka Hao, Tomohiko Shindo, Tsuyoshi Ogata, Yuta Kagaya, Ryo Kurosawa, Kensuke Nishimiya, Kimio Satoh, Satoshi Miyata, Kazuyoshi Kawakami, Hiroaki Shimokawa

Author information

Yuzuru Abe
Department of Cardiovascular Medicine, Tohoku University Graduate School of Medicine
Department of Medical Microbiology, Mycology and Immunology, Tohoku University Graduate School of Medicine
Kenta Ito
Department of Cardiovascular Medicine, Tohoku University Graduate School of Medicine
Kiyotaka Hao
Department of Cardiovascular Medicine, Tohoku University Graduate School of Medicine
Tomohiko Shindo
Department of Cardiovascular Medicine, Tohoku University Graduate School of Medicine
Tsuyoshi Ogata
Department of Cardiovascular Medicine, Tohoku University Graduate School of Medicine
Yuta Kagaya
Department of Cardiovascular Medicine, Tohoku University Graduate School of Medicine
Ryo Kurosawa
Department of Cardiovascular Medicine, Tohoku University Graduate School of Medicine
Kensuke Nishimiya
Department of Cardiovascular Medicine, Tohoku University Graduate School of Medicine
Kimio Satoh
Department of Cardiovascular Medicine, Tohoku University Graduate School of Medicine
Satoshi Miyata
Department of Cardiovascular Medicine, Tohoku University Graduate School of Medicine
Kazuyoshi Kawakami
Department of Medical Microbiology, Mycology and Immunology, Tohoku University Graduate School of Medicine
Hiroaki Shimokawa
Department of Cardiovascular Medicine, Tohoku University Graduate School of Medicine

Keywords: Inflammation, Left ventricular remodeling, Macrophages, Myocardial infarction, Shock-wave therapy

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Supplementary material

2014 Volume 78 Issue 12 Pages 2915-2925

DOI https://doi.org/10.1253/circj.CJ-14-0230

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Abstract

Background:It has been previously demonstrated that extracorporeal low-energy shock-wave (SW) therapy ameliorates left ventricular (LV) remodeling through enhanced angiogenesis after acute myocardial infarction (AMI) in pigs in vivo. However, it remains to be examined whether SW therapy also exerts anti-inflammatory effects on AMI.Methods and Results:AMI was created by ligating the proximal left anterior descending coronary artery in rats. They were randomly assigned to 2 groups: with (SW group) or without (control group) SW therapy (0.1 mJ/mm², 200 shots, 1 Hz to the whole heart at 1, 3 and 5 days after AMI). Four weeks after AMI, SW therapy significantly ameliorated LV remodeling and fibrosis. Histological examinations showed that SW therapy significantly suppressed the infiltration of neutrophils and macrophages at days 3 and 6, in addition to enhanced capillary density in the border area. Molecular examinations demonstrated that SW therapy enhanced the expression of endothelial nitric oxide synthase and suppressed the infiltration of transforming growth factor-β1-positive cells early after AMI. SW therapy also upregulated anti-inflammatory cytokines and downregulated pro-inflammatory cytokines in general.Conclusions:These results suggest that low-energy SW therapy suppressed post-MI LV remodeling in rats in vivo, which was associated with anti-inflammatory effects in addition to its angiogenic effects, and demonstrated a novel aspect of the therapy for AMI. (Circ J 2014; 78: 2915–2925)

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