In vitro osteoinduction of human mesenchymal stem cells in biomimetic surface modified titanium alloy implants

Sonia SANTANDER; Clara ALCAINE; Jaber LYAHYAI; Maria Angeles PÉREZ; Clementina RODELLAR; Manuel DOBLARÉ; Ignacio OCHOA

doi:10.4012/dmj.2012-015-r

Original Paper

In vitro osteoinduction of human mesenchymal stem cells in biomimetic surface modified titanium alloy implants

Sonia SANTANDER, Clara ALCAINE, Jaber LYAHYAI, Maria Angeles PÉREZ, Clementina RODELLAR, Manuel DOBLARÉ, Ignacio OCHOA

Author information

Sonia SANTANDER
Biomedical Research Networking Center in Bioengineering, Biomaterials and Nanomedicine (CIBER-BBN)
Group of Structural Mechanics and Materials Modelling (GEMM), Aragón Institute of Engineering Research, University of Zaragoza
Carlos III Health Institute (ISCIII), Aragón Institute of Health Sciences
Clara ALCAINE
Biomedical Research Networking Center in Bioengineering, Biomaterials and Nanomedicine (CIBER-BBN)
Group of Structural Mechanics and Materials Modelling (GEMM), Aragón Institute of Engineering Research, University of Zaragoza
Carlos III Health Institute (ISCIII), Aragón Institute of Health Sciences
Jaber LYAHYAI
Carlos III Health Institute (ISCIII), Aragón Institute of Health Sciences
Lagenbio-Ingen, Aragón Institute of Engineering Research, University of Zaragoza
Maria Angeles PÉREZ
Biomedical Research Networking Center in Bioengineering, Biomaterials and Nanomedicine (CIBER-BBN)
Group of Structural Mechanics and Materials Modelling (GEMM), Aragón Institute of Engineering Research, University of Zaragoza
Carlos III Health Institute (ISCIII), Aragón Institute of Health Sciences
Clementina RODELLAR
Carlos III Health Institute (ISCIII), Aragón Institute of Health Sciences
Lagenbio-Ingen, Aragón Institute of Engineering Research, University of Zaragoza
Manuel DOBLARÉ
Biomedical Research Networking Center in Bioengineering, Biomaterials and Nanomedicine (CIBER-BBN)
Group of Structural Mechanics and Materials Modelling (GEMM), Aragón Institute of Engineering Research, University of Zaragoza
Carlos III Health Institute (ISCIII), Aragón Institute of Health Sciences
Ignacio OCHOA
Biomedical Research Networking Center in Bioengineering, Biomaterials and Nanomedicine (CIBER-BBN)
Group of Structural Mechanics and Materials Modelling (GEMM), Aragón Institute of Engineering Research, University of Zaragoza
Carlos III Health Institute (ISCIII), Aragón Institute of Health Sciences

Keywords: Osteoinduction, Biomimetic surface, Titanium implant, Human mesenchymal stem cells, Combined surface treatment

JOURNAL FREE ACCESS

2014 Volume 33 Issue 3 Pages 305-312

DOI https://doi.org/10.4012/dmj.2012-015-r

Details

Abstract

Interaction between cells and implant surface is crucial for clinical success. This interaction and the associated surface treatment are essential for achieving a fast osseointegration process. Several studies of different topographical or chemical surface modifications have been proposed previously in literature. The Biomimetic Advanced Surface (BAS) topography is a combination of a shot blasting and anodizing procedure. Macroroughness, microporosity of titanium oxide and Calcium/Phosphate ion deposition is obtained. Human mesenchymal stem cells (hMCSs) response in vitro to this treatment has been evaluated. The results obtained show an improved adhesion capacity and a higher proliferation rate when hMSCs are cultured on treated surfaces. This biomimetic modification of the titanium surface induces the expression of osteblastic differentiation markers (RUNX2 and Osteopontin) in the absence of any externally provided differentiation factor. As a main conclusion, our biomimetic surface modification could lead to a substantial improvement in osteoinduction in titanium alloy implants.

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