Development of non toxic magnetic beads has been strongly desired to cure more effectively for patients having diseases such as cancer and osteosarcoma. In this study, Fe was added during calcium phosphate preparation by conventional wet process, and the condition of Fe ion and the effect of Fe doping on the crystal phase, microstructure and other properties of HAp were investigated. The crystal phase of obtained samples had a HAp structure with low crystallinity, and the crystallinity of samples decreased with increasing the Fe ratio. The obtained powders had granular crystals with 10 nm in diameter. From the XRD and XANES measurement, it is thought that the actual doped amount into the HAp structure was 1, 1.8 and 3 mol % for Ca of HAp in the case of 5, 10 and 20 mol % of addition, respectively, and the most of added Fe existed as an iron oxide phase at the surface of HAp particles.