Construction of Swine-Specific CpG Motif Enriched Plasmid and the Study of Its Immunostimulatory Effects Both In Vitro and In Vivo

Xiaoyu GUO; Hong JIA; Weifeng YUAN; Quan ZHANG; Shaohua HOU; Yao SUN; Gang ZHU; Hongfei ZHU; José Manuel SÁNCHEZ-VIZCAÍNO

doi:10.1292/jvms.11-0543

Immunology

Construction of Swine-Specific CpG Motif Enriched Plasmid and the Study of Its Immunostimulatory Effects Both In Vitro and In Vivo

Xiaoyu GUO, Hong JIA, Weifeng YUAN, Quan ZHANG, Shaohua HOU, Yao SUN, Gang ZHU, Hongfei ZHU, José Manuel SÁNCHEZ-VIZCAÍNO

Author information

Xiaoyu GUO
Institute of Animal Science, Chinese Academy of Agricultural Sciences, Beijing 100081, China
Centro de Vigilancia Sanitaria Veterinaria (VISAVET) and Animal Health Department, Complutense University of Madrid, Avda. Puerta de Hierro, S/N 28040 Madrid, Spain
Hong JIA
Institute of Animal Science, Chinese Academy of Agricultural Sciences, Beijing 100081, China
Weifeng YUAN
Institute of Animal Science, Chinese Academy of Agricultural Sciences, Beijing 100081, China
Quan ZHANG
College of Veterinary Medicine, Yangzhou University, Yangzhou 225009, China
Shaohua HOU
Institute of Animal Science, Chinese Academy of Agricultural Sciences, Beijing 100081, China
Yao SUN
China Animal Husbandry Group Research Institute, Beijing 100081, China
Gang ZHU
Xinjiang Tiankang Animal Science Bio-Technology Co. Ltd., Urumqi, 830000, China
Hongfei ZHU
Institute of Animal Science, Chinese Academy of Agricultural Sciences, Beijing 100081, China
José Manuel SÁNCHEZ-VIZCAÍNO
Centro de Vigilancia Sanitaria Veterinaria (VISAVET) and Animal Health Department, Complutense University of Madrid, Avda. Puerta de Hierro, S/N 28040 Madrid, Spain

Keywords: antibody titer, CpG motif, FMD killed vaccine, lymphocyte transformation test, stimulation Index

JOURNAL FREE ACCESS

2012 Volume 74 Issue 12 Pages 1647-1650

DOI https://doi.org/10.1292/jvms.11-0543

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Abstract

A swine-specific CpG motif enriched plasmid (pUC18-CpG) was constructed in this study. Its immunostimulant property was tested in vitro via lymphocyte transformation assay using swine peripheral blood mononuclear cells (PBMCs). The recombinant plasmid showed higher Stimulation Index (SI) compared to the positive control (LPS). In a following animal experiment, pUC18-CpG was co-administered with a commercial swine FMD killed vaccine. Animals in the pUC18-CpG adjuvanted groups showed much higher antibody titers during the vaccination period.

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