Establishment of Five Canine Lymphoma Cell Lines and Tumor Formation in a Xenotransplantation Model

Saori UMEKI; Yasuo EMA; Ryoichi SUZUKI; Masahito KUBO; Toshiharu HAYASHI; Yasuhiko OKAMURA; Jumpei YAMAZAKI; Hajime TSUJIMOTO; Kenji TANI; Hiroko HIRAOKA; Masaru OKUDA; Takuya MIZUNO

doi:10.1292/jvms.12-0448

Internal Medicine

Establishment of Five Canine Lymphoma Cell Lines and Tumor Formation in a Xenotransplantation Model

Saori UMEKI, Yasuo EMA, Ryoichi SUZUKI, Masahito KUBO, Toshiharu HAYASHI, Yasuhiko OKAMURA, Jumpei YAMAZAKI, Hajime TSUJIMOTO, Kenji TANI, Hiroko HIRAOKA, Masaru OKUDA, Takuya MIZUNO

Author information

Saori UMEKI
Laboratory of Veterinary Internal Medicine, The United Graduate School of Veterinary Science, Yamaguchi University, 1677–1 Yoshida, Yamaguchi 753–8515, Japan
Yasuo EMA
Laboratory of Veterinary Internal Medicine, Joint Faculty of Veterinary Medicine, Yamaguchi University, 1677–1 Yoshida, Yamaguchi 753–8515, Japan
Ryoichi SUZUKI
Laboratory of Veterinary Internal Medicine, Joint Faculty of Veterinary Medicine, Yamaguchi University, 1677–1 Yoshida, Yamaguchi 753–8515, Japan
Masahito KUBO
Laboratory of Veterinary Pathology, Joint Faculty of Veterinary Medicine, Yamaguchi University, 1677–1 Yoshida, Yamaguchi 753–8515, Japan
Toshiharu HAYASHI
Laboratory of Veterinary Pathology, Joint Faculty of Veterinary Medicine, Yamaguchi University, 1677–1 Yoshida, Yamaguchi 753–8515, Japan
Yasuhiko OKAMURA
Laboratory of Veterinary Surgery, Department of Veterinary Medicine, Faculty of Agriculture, Iwate University, 3–18–8 Ueda, Morioka, Iwate 020–8550, Japan
Jumpei YAMAZAKI
Fels Institute for Cancer Research and Molecular Biology, Temple University School of Medicine, Philadelphia, Pennsylvania, U.S.A.
Hajime TSUJIMOTO
Laboratory of Veterinary Internal Medicine, Graduate School of Agricultural and Life Sciences, The University of Tokyo, 1–1–1 Yayoi, Bunkyo-ku, Tokyo 113–8657, Japan
Kenji TANI
Laboratory of Veterinary Surgery, Joint Faculty of Veterinary Medicine, Yamaguchi University, 1677–1 Yoshida, Yamaguchi 753–8515, Japan
Hiroko HIRAOKA
Laboratory of Veterinary Clinical Pathology, Joint Faculty of Veterinary Medicine, Yamaguchi University, 1677–1 Yoshida, Yamaguchi 753–8515, Japan
Masaru OKUDA
Laboratory of Veterinary Internal Medicine, The United Graduate School of Veterinary Science, Yamaguchi University, 1677–1 Yoshida, Yamaguchi 753–8515, Japan
Laboratory of Veterinary Internal Medicine, Joint Faculty of Veterinary Medicine, Yamaguchi University, 1677–1 Yoshida, Yamaguchi 753–8515, Japan
Biomedical Science Center for Translational Research, The United Graduate School of Veterinary Science, Yamaguchi University, 1677–1 Yoshida, Yamaguchi 753–8515, Japan
Takuya MIZUNO
Laboratory of Veterinary Internal Medicine, The United Graduate School of Veterinary Science, Yamaguchi University, 1677–1 Yoshida, Yamaguchi 753–8515, Japan
Laboratory of Veterinary Internal Medicine, Joint Faculty of Veterinary Medicine, Yamaguchi University, 1677–1 Yoshida, Yamaguchi 753–8515, Japan
Biomedical Science Center for Translational Research, The United Graduate School of Veterinary Science, Yamaguchi University, 1677–1 Yoshida, Yamaguchi 753–8515, Japan

Keywords: canine lymphoma, cell line, characterization, xenograft model

JOURNAL FREE ACCESS

2013 Volume 75 Issue 4 Pages 467-474

DOI https://doi.org/10.1292/jvms.12-0448

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Abstract

Five novel, canine lymphoma cell lines (Ema, CLC, CLK, Nody-1 and UL-1) were established from dogs suffering from lymphoma and characterized in vitro and in vivo. All cell lines, except CLC, were characterized with T-cell phenotypes, by flow cytometric analysis and polymerase chain reaction for antigen receptor rearrangement. Cell proliferation rates and transcriptional levels of MYC, PTEN, KIT and FLT3 varied between each cell line. Intraperitoneal xenotransplantation of Ema, CLC, Nody-1 and UL-1 lymphoma cell lines into NOD/SCID mice induced ascites, intraperitoneal tumors and severe infiltration of lymphoma cells into the pancreas and mesentery. Establishment of novel canine lymphoma cell lines with different characteristics is critical for elucidating the pathophysiology of canine lymphoma and improving current therapies.

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