Cross-Reactivity of Secondary Antibodies against African Rodents and Application for Sero-Surveillance

Ichiro NAKAMURA; Bernard Mudenda HANG’OMBE; Hirofumi SAWA; Shintaro KOBAYASHI; Yasuko ORBA; Akihiro ISHII; Yuka THOMAS; Rie ISOZUMI; Kumiko YOSHIMATSU; Aaron S. MWEENE; Ayato TAKADA; Chihiro SUGIMOTO; Jiro ARIKAWA

doi:10.1292/jvms.12-0471

Public Health

Cross-Reactivity of Secondary Antibodies against African Rodents and Application for Sero-Surveillance

Ichiro NAKAMURA, Bernard Mudenda HANG’OMBE, Hirofumi SAWA, Shintaro KOBAYASHI, Yasuko ORBA, Akihiro ISHII, Yuka THOMAS, Rie ISOZUMI, Kumiko YOSHIMATSU, Aaron S. MWEENE, Ayato TAKADA, Chihiro SUGIMOTO, Jiro ARIKAWA

Author information

Ichiro NAKAMURA
Hokkaido University Research Center for Zoonosis Control, N20W10, Kita-ku, Sapporo, Hokkaido 001–0020, Japan
School of Veterinary Medicine, University of Zambia, P. O. Box 32379, Lusaka, Zambia
Bernard Mudenda HANG’OMBE
School of Veterinary Medicine, University of Zambia, P. O. Box 32379, Lusaka, Zambia
Hirofumi SAWA
Hokkaido University Research Center for Zoonosis Control, N20W10, Kita-ku, Sapporo, Hokkaido 001–0020, Japan
School of Veterinary Medicine, University of Zambia, P. O. Box 32379, Lusaka, Zambia
Shintaro KOBAYASHI
Hokkaido University Research Center for Zoonosis Control, N20W10, Kita-ku, Sapporo, Hokkaido 001–0020, Japan
Yasuko ORBA
Hokkaido University Research Center for Zoonosis Control, N20W10, Kita-ku, Sapporo, Hokkaido 001–0020, Japan
Akihiro ISHII
Hokkaido University Research Center for Zoonosis Control, N20W10, Kita-ku, Sapporo, Hokkaido 001–0020, Japan
School of Veterinary Medicine, University of Zambia, P. O. Box 32379, Lusaka, Zambia
Yuka THOMAS
Hokkaido University Research Center for Zoonosis Control, N20W10, Kita-ku, Sapporo, Hokkaido 001–0020, Japan
School of Veterinary Medicine, University of Zambia, P. O. Box 32379, Lusaka, Zambia
Rie ISOZUMI
Graduate School of Medicine, Hokkaido University, N15W7, Kita-ku, Sapporo, Hokkaido 060–8638, Japan
Kumiko YOSHIMATSU
Graduate School of Medicine, Hokkaido University, N15W7, Kita-ku, Sapporo, Hokkaido 060–8638, Japan
Aaron S. MWEENE
School of Veterinary Medicine, University of Zambia, P. O. Box 32379, Lusaka, Zambia
Ayato TAKADA
Hokkaido University Research Center for Zoonosis Control, N20W10, Kita-ku, Sapporo, Hokkaido 001–0020, Japan
School of Veterinary Medicine, University of Zambia, P. O. Box 32379, Lusaka, Zambia
Chihiro SUGIMOTO
Hokkaido University Research Center for Zoonosis Control, N20W10, Kita-ku, Sapporo, Hokkaido 001–0020, Japan
School of Veterinary Medicine, University of Zambia, P. O. Box 32379, Lusaka, Zambia
Jiro ARIKAWA
Graduate School of Medicine, Hokkaido University, N15W7, Kita-ku, Sapporo, Hokkaido 060–8638, Japan

Keywords: hantavirus, leptospirosis, plague, rodent-borne zoonoses, sero-surveillance

JOURNAL FREE ACCESS

2013 Volume 75 Issue 6 Pages 819-825

DOI https://doi.org/10.1292/jvms.12-0471

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Abstract

A total of 466 rodents were captured in the Republic of Zambia from 2006 to 2010. Based on morphological observations and phylogenetic analyses of mitochondrial gene sequences, rodents were divided into 10 groups consisting of 39 Rattus rodents, 263 multimammate rats, 18 other Murinae rodents, 95 gerbils, 11 pouched mice, 1 giant-pouched rat, 38 fat mice and 1 dormouse. Rodent antibodies except that from Rattus were examined for their cross-reactivity to commercially available antibody detection reagents. Anti-mouse immunoglobulin G (IgG) was most cross-reactive to heterologous antibodies including multimammate rat, gerbil, pouched mouse and fat mouse. Thus, anti-mouse IgG would be a useful detection tool in serological examination of the Zambian rodent population. Preliminary sero-surveillance for plague, leptospirosis and hantavirus infection was performed by ELISA.

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