Interlaboratory Study of LC-UV and LC-MS Methods for the Simultaneous Determination of Deoxynivalenol and Nivalenol in Wheat

Koji AOYAMA; Hajime AKASHI; Naoki MOCHIZUKI; Yuji ITO; Takashi MIYASHITA; Soohyung LEE; Motoki OGISO; Mamoru MAEDA; Shigemi KAI; Hiroki TANAKA; Hiroko NORIDUKI; Hisaaki HIRAOKA; Toshitsugu TANAKA; Eiichi ISHIKURO; Yoshinori ITOH; Toshihiro NAGAYAMA; Masahiro NAKAJIMA; Shigehiro NAITO; Yoshiko SUGITA-KONISHI

doi:10.3358/shokueishi.53.152

Validation Study

Interlaboratory Study of LC-UV and LC-MS Methods for the Simultaneous Determination of Deoxynivalenol and Nivalenol in Wheat

Koji AOYAMA, Hajime AKASHI, Naoki MOCHIZUKI, Yuji ITO, Takashi MIYASHITA, Soohyung LEE, Motoki OGISO, Mamoru MAEDA, Shigemi KAI, Hiroki TANAKA, Hiroko NORIDUKI, Hisaaki HIRAOKA, Toshitsugu TANAKA, Eiichi ISHIKURO, Yoshinori ITOH, Toshihiro NAGAYAMA, Masahiro NAKAJIMA, Shigehiro NAITO, Yoshiko SUGITA-KONISHI

Author information

Koji AOYAMA
Food and Agricultural Materials Inspection Center
Hajime AKASHI
Nisshin Seifun Group Inc.
Naoki MOCHIZUKI
Asahi Breweries Ltd.
Yuji ITO
Kirin Group Office Company Ltd.
Takashi MIYASHITA
Kewpie Corporation
Soohyung LEE
Rural Development Administration
Motoki OGISO
Japan Food Research Laboratories
Mamoru MAEDA
Japan Frozen Foods Inspection Corporation
Shigemi KAI
Kanagawa Prefectural Institute of Public Health
Hiroki TANAKA
Suntory Business Expert Ltd.
Hiroko NORIDUKI
Japan Grain Inspection Association
Hisaaki HIRAOKA
Food and Agricultural Materials Inspection Center
Toshitsugu TANAKA
Kobe Institute of Health
Eiichi ISHIKURO
Japan Food Research Laboratories
Yoshinori ITOH
National Institute of Health Sciences
Toshihiro NAGAYAMA
Tokyo Metropolitan Institute of Public Health
Masahiro NAKAJIMA
Nagoya City Public Health Research Institute
Shigehiro NAITO
National Food Research Institute
Yoshiko SUGITA-KONISHI
National Institute of Health Sciences

Keywords: deoxynivalenol, nivalenol, liquid chromatography (LC), ultraviolet (UV) detector, mass spectrometric (MS) detector, interlaboratory study, wheat

JOURNAL FREE ACCESS

2012 Volume 53 Issue 3 Pages 152-156

DOI https://doi.org/10.3358/shokueishi.53.152

Details

Abstract

To evaluate LC methods with UV or MS detection for simultaneous analysis of deoxynivalenol (DON) and nivalenol (NIV) in wheat, an interlaboratory study was conducted in 11 laboratories. DON and NIV were purified using a multifunctional column, and their concentrations were determined using LC-UV or LC-MS(/MS). No internal standards were used. Three fortified wheat samples (0.1, 0.5 and 1 mg/kg), one naturally contaminated wheat sample, and one blank wheat sample were used. The recoveries ranged from 90% to 110% for DON and from 76% to 83% for NIV. For DON, the relative standard deviations for repeatability (RSDr) ranged from 1.1% to 7.6%. The relative standard deviations for reproducibility (RSDr) ranged from 7.2% to 25.2%. For NIV, the RSDr ranged from 2.0% to 10.7%, and the RSDr ranged from 7.0% to 31.4%. Regardless of sample and detector, the HorRat values for DON and NIV ranged from 0.4 to 1.4. Both LC-UV and LC-MS(/MS) methods were considered to be suitable for application as an official method.

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