No Increase of Large-scale Mitochondrial DNA Deletions in Peripheral Blood Cells in Residents of Kazakhstan around Semipalatinsk Nuclear Test Site

Aiko HAMADA; Neilya J. CHAIZHUNUSOVA; Vladimir A. SAENKO; Tatiana I. ROGOUNOVITCH; Noboru TAKAMURA; Hiroyuki NAMBA; Kazbek N. APSALIKOV; Shunichi YAMASHITA

doi:10.11343/amn.49.45

ORIGINAL ARTICLES

No Increase of Large-scale Mitochondrial DNA Deletions in Peripheral Blood Cells in Residents of Kazakhstan around Semipalatinsk Nuclear Test Site

Aiko HAMADA, Neilya J. CHAIZHUNUSOVA, Vladimir A. SAENKO, Tatiana I. ROGOUNOVITCH, Noboru TAKAMURA, Hiroyuki NAMBA, Kazbek N. APSALIKOV, Shunichi YAMASHITA

Author information

Aiko HAMADA
Department of Molecular Medicine, Atomic Bomb Disease Institute, Nagasaki University Graduate School of Biomedical Sciences
Neilya J. CHAIZHUNUSOVA
Kazakhstan Research Institute of Radiation Medicine and Ecology
Vladimir A. SAENKO
Department of International Health and Radiation Research, Atomic Bomb Disease Institute, Nagasaki University Graduate School of Biomedical Sciences
Tatiana I. ROGOUNOVITCH
Department of Molecular Medicine, Atomic Bomb Disease Institute, Nagasaki University Graduate School of Biomedical Sciences
Noboru TAKAMURA
Department of Public Health, Nagasaki University Graduate School of Biomedical Sciences
Hiroyuki NAMBA
Department of Molecular Medicine, Atomic Bomb Disease Institute, Nagasaki University Graduate School of Biomedical Sciences
Kazbek N. APSALIKOV
Kazakhstan Research Institute of Radiation Medicine and Ecology
Shunichi YAMASHITA
Department of Molecular Medicine, Atomic Bomb Disease Institute, Nagasaki University Graduate School of Biomedical Sciences
Department of International Health and Radiation Research, Atomic Bomb Disease Institute, Nagasaki University Graduate School of Biomedical Sciences

Keywords: Mitochondrial DNA, Radiation, Peripheral blood lymphocytes, Semipalatinsk Nuclear Test Site

JOURNAL FREE ACCESS

2004 Volume 49 Issue 1+2 Pages 45-49

DOI https://doi.org/10.11343/amn.49.45

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Abstract

The study was set out to elucidate whether there are detectable differences in the molecular features of mitochondrial DNA (mtDNA) from peripheral blood lymphocytes (PBLs) between individuals possibly exposed to radiation due to fallouts from Semipalatinsk Nuclear Test Site and those who lived in non-contaminated area in Kazakhstan. Subjects were residents of three villages in Kazakhstan considered to be affected with radionuclides in the past. Control individuals were from one non-contaminated settlement in Kazakhstan and age-matched Japanese volunteers. Two parameters, the relative mtDNA content and abundance of large-scale deletions (LSDs) in mtDNA were estimated by real-time PCR and a PCR adjusted to sensitively reveal the LSDs, respectively. Relative content of mtDNA as well as the abundance of LSDs were found to differ non-significantly both in Kazakhstan radiation exposed, control Kazakhstan and Japanese individuals. LSD scores displayed no regular or statistically significant association with the mtDNA content in any group tested. Lack of correlation between the number of LSDs and mtDNA level occurred irrespectively the radiation history of a blood donor or individual's age. We conclude that the molecular parameters of PBL mtDNA measured in this work are unlikely to be informative as a bioindicator of radiation exposure.

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