The Bovine Lactophorin C-Terminal Fragment and PAS6/7 Were Both Potent in the Inhibition of Human Rotavirus Replication in Cultured Epithelial Cells and the Prevention of Experimental Gastroenteritis

Mizuho INAGAKI; Sayaka NAGAI; Tomio YABE; Satoshi NAGAOKA; Nobuyuki MINAMOTO; Takeshi TAKAHASHI; Tsukasa MATSUDA; Osamu NAKAGOMI; Toyoko NAKAGOMI; Takusaburo EBINA; Yoshihiro KANAMARU

doi:10.1271/bbb.100060

Food & Nutrition Science Regular Papers

The Bovine Lactophorin C-Terminal Fragment and PAS6/7 Were Both Potent in the Inhibition of Human Rotavirus Replication in Cultured Epithelial Cells and the Prevention of Experimental Gastroenteritis

Mizuho INAGAKI, Sayaka NAGAI, Tomio YABE, Satoshi NAGAOKA, Nobuyuki MINAMOTO, Takeshi TAKAHASHI, Tsukasa MATSUDA, Osamu NAKAGOMI, Toyoko NAKAGOMI, Takusaburo EBINA, Yoshihiro KANAMARU

Author information

Mizuho INAGAKI
United Graduate School of Agricultural Science, Gifu University
Sayaka NAGAI
Department of Applied Life Science, Gifu University
Tomio YABE
Department of Applied Life Science, Gifu University
Satoshi NAGAOKA
Department of Applied Life Science, Gifu University
Nobuyuki MINAMOTO
Veterinary Medicine, Faculty of Applied Biological Sciences, Gifu University
Takeshi TAKAHASHI
Food Science Institute, Division of Research and Development, Meiji Dairies Co., Ltd.
Tsukasa MATSUDA
Department of Applied Molecular Biosciences, Graduate School of Bioagricultural Sciences, Nagoya University
Osamu NAKAGOMI
Department of Molecular Microbiology and Immunology, Graduate School of Biomedical Sciences and Global Center of Excellence, Nagasaki University
Toyoko NAKAGOMI
Department of Molecular Microbiology and Immunology, Graduate School of Biomedical Sciences and Global Center of Excellence, Nagasaki University
Takusaburo EBINA
Sendai Institute of Microbiology
Yoshihiro KANAMARU
Department of Applied Life Science, Gifu University

Keywords: human rotavirus, diarrhea, bovine milk, lactophorin, PAS6/7

JOURNAL FREE ACCESS

2010 Volume 74 Issue 7 Pages 1386-1390

DOI https://doi.org/10.1271/bbb.100060

View "Advance Publication" version (July 7, 2010).

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Abstract

Rotaviruses are the leading cause of severe dehydrating diarrhea in children worldwide. We have found that high-M_r glycoprotein fraction (F1) from cow’s milk whey has potent inhibitory activity against human rotavirus (HRV) in cell culture. The present study was undertaken to identify and characterize the components responsible for this inhibitory activity. F1 was initially heated at 95 °C for 30 min, rendering milk antibodies inert, subjected to ammonium sulfate fractionation, and then resolved by two-dimensional polyacrylamide gel electrophoresis. After electroelution, we found that a heat-stable milk protein lactophorin C-terminal fragment (LP16) and bovine milk fat globule membrane protein PAS6/7 strongly inhibited the replication of HRV MO strains in MA104 cells. Furthermore, we found that prophylactic oral administration of F1 once before inoculation of the HRV MO strain obviously prevented the development of diarrhea in vivo. These non-immunoglobulin components are a promising candidate for a prophylactic food additive against HRV infection.

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