Heterologous Expression of Aspergillus oryzae Xylose Reductase and Xylitol Dehydrogenase Genes Facilitated Xylose Utilization in the Yeast Saccharomyces cerevisiae

Jitsuro KANEDA; Kenji SASAKI; Katsuya GOMI; Takahiro SHINTANI

doi:10.1271/bbb.100639

Microbiology & Fermentation Technology Notes

Heterologous Expression of Aspergillus oryzae Xylose Reductase and Xylitol Dehydrogenase Genes Facilitated Xylose Utilization in the Yeast Saccharomyces cerevisiae

Jitsuro KANEDA, Kenji SASAKI, Katsuya GOMI, Takahiro SHINTANI

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Keywords: Aspergillus oryzae, Saccharomyces cerevisiae, xylose fermentation, xylose reductase, ethanol

JOURNAL FREE ACCESS

2011 Volume 75 Issue 1 Pages 168-170

DOI https://doi.org/10.1271/bbb.100639

View "Advance Publication" version (January 7, 2011).

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Abstract

The cDNA encoding a putative xylose reductase (xyrA) from Aspergillus oryzae was cloned and coexpressed in the yeast Saccharomyces cerevisiae with A. oryzae xylitol dehydrogenase cDNA (xdhA). XyrA exhibited NADPH-dependent xylose reductase activity. The S. cerevisiae strain, overexpressing the xyrA, xdhA, endogenous XKS1, and TAL1 genes, grew on xylose as sole carbon source, and produced ethanol.

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