FXYD6, a Na,K-ATPase Regulator, Is Expressed in Type II Taste Cells

Yoichiro SHINDO; Kana MORISHITA; Eiichi KOTAKE; Hirohito MIURA; Piero CARNINCI; Jun KAWAI; Yoshihide HAYASHIZAKI; Akihiro HINO; Tomomasa KANDA; Yuko KUSAKABE

doi:10.1271/bbb.100718

Biochemistry & Molecular Biology Regular Papers

FXYD6, a Na,K-ATPase Regulator, Is Expressed in Type II Taste Cells

Yoichiro SHINDO, Kana MORISHITA, Eiichi KOTAKE, Hirohito MIURA, Piero CARNINCI, Jun KAWAI, Yoshihide HAYASHIZAKI, Akihiro HINO, Tomomasa KANDA, Yuko KUSAKABE

Author information

Yoichiro SHINDO
Research Laboratories for Health and Gustatory Science, Asahi Breweries, Ltd.
Kana MORISHITA
National Food Research Institute, National Agriculture and Food Research Organization
Eiichi KOTAKE
Research Laboratories for Health and Gustatory Science, Asahi Breweries, Ltd.
Hirohito MIURA
Kagoshima University Graduate School of Medical and Dental Sciences
Piero CARNINCI
RIKEN Omics Sciences Center
Jun KAWAI
RIKEN Omics Sciences Center
Yoshihide HAYASHIZAKI
RIKEN Omics Sciences Center
Akihiro HINO
National Food Research Institute, National Agriculture and Food Research Organization
Tomomasa KANDA
Research Laboratories for Health and Gustatory Science, Asahi Breweries, Ltd.
Yuko KUSAKABE
National Food Research Institute, National Agriculture and Food Research Organization

Keywords: Na,K-ATPase, taste cells, gene expression pattern, in situ hybridization, regulation of sodium ions

JOURNAL FREE ACCESS

2011 Volume 75 Issue 6 Pages 1061-1066

DOI https://doi.org/10.1271/bbb.100718

View "Advance Publication" version (June 13, 2011).

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Abstract

Taste buds contain three types of taste cells. Each type can respond to taste stimulation, and type II and III taste cells are electrically excitable. However, there are differences between the properties of type II and III taste cells. In this study, we found that Fxyd6, an Na,K-ATPase regulator gene, is expressed in type II taste cells in the taste buds of mice. Double-labeled in situ hybridization analysis showed that Fxyd6 was coexpressed with transient receptor potential cation channel, subfamily M, member 5 (Trpm5), a critical component of the sweet, bitter, and umami taste signal transduction pathways and that it was specifically expressed in type II taste cells. We also found that taste cells frequently coexpressed Fxyd6 and Na,K-ATPase β1. These results indicate the presence of an inherent mechanism that regulated transmembrane Na⁺ dynamics in type II taste cells.

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