Calreticulin Inhibits Prion Protein PrP-(23–98) Aggregation in Vitro

Noriyuki SHIRAISHI; Yoko INAI; Yoshiaki HIRANO; Yoshito IHARA

doi:10.1271/bbb.110287

Biochemistry & Molecular Biology Notes

Calreticulin Inhibits Prion Protein PrP-(23–98) Aggregation in Vitro

Noriyuki SHIRAISHI, Yoko INAI, Yoshiaki HIRANO, Yoshito IHARA

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Keywords: prion protein, PrP-(23–98), aggregates, calreticulin

JOURNAL FREE ACCESS

2011 Volume 75 Issue 8 Pages 1625-1627

DOI https://doi.org/10.1271/bbb.110287

View "Advance Publication" version (August 7, 2011).

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Abstract

Because prion protein PrP-(23–98) was recently found to polymerize into amyloid-like and proteinase K-resistant spherical aggregates in the presence of NADPH plus copper ions, we tested to determine whether calreticulin (CRT) inhibits PrP-(23–98) aggregation in vitro. The results indicated that CRT suppressed PrP-(23–98) aggregation, and that CRT-mediated solubilization occurred in the aggregates.

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