Two-Dimensional Protein Separation by the HPLC System with a Monolithic Column

Hironobu MORISAKA; Aya KIRINO; Kengo KOBAYASHI; Mitsuyoshi UEDA

doi:10.1271/bbb.110770

Analytical Chemistry Note

Two-Dimensional Protein Separation by the HPLC System with a Monolithic Column

Hironobu MORISAKA, Aya KIRINO, Kengo KOBAYASHI, Mitsuyoshi UEDA

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Keywords: multi-dimensional high-performance liquid chromatography, monolithic column, proteomic analysis, protein separation, yeast cell surface

JOURNAL FREE ACCESS

2012 Volume 76 Issue 3 Pages 585-588

DOI https://doi.org/10.1271/bbb.110770

View "Advance Publication" version (March 7, 2012).

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Abstract

A two-dimensional high-performance liquid chromatography (2D-HPLC) system for protein separation was developed using an ion-exchange column in the first dimension and a reversed-phase monolithic column in the second dimension. The system demonstrated efficient separation of proteins in comparison with conventional systems. For proteomic analysis, proteins extracted from the cell surface of the yeast were separated by 2D-HPLC and evaluated.

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