Efficient Production of 2-Deoxyribose 5-Phosphate from Glucose and Acetaldehyde by Coupling of the Alcoholic Fermentation System of Baker’s Yeast and Deoxyriboaldolase-Expressing Escherichia coli

Nobuyuki HORINOUCHI; Jun OGAWA; Takako KAWANO; Takafumi SAKAI; Kyota SAITO; Seiichiro MATSUMOTO; Mie SASAKI; Yoichi MIKAMI; Sakayu SHIMIZU

doi:10.1271/bbb.50648

Microbiology & Fermentation Technology Regular Papers

Efficient Production of 2-Deoxyribose 5-Phosphate from Glucose and Acetaldehyde by Coupling of the Alcoholic Fermentation System of Baker’s Yeast and Deoxyriboaldolase-Expressing Escherichia coli

Nobuyuki HORINOUCHI, Jun OGAWA, Takako KAWANO, Takafumi SAKAI, Kyota SAITO, Seiichiro MATSUMOTO, Mie SASAKI, Yoichi MIKAMI, Sakayu SHIMIZU

Author information

Nobuyuki HORINOUCHI
Division of Applied Life Sciences, Graduate School of Agriculture, Kyoto University
Jun OGAWA
Division of Applied Life Sciences, Graduate School of Agriculture, Kyoto University
Takako KAWANO
Division of Applied Life Sciences, Graduate School of Agriculture, Kyoto University
Takafumi SAKAI
Division of Applied Life Sciences, Graduate School of Agriculture, Kyoto University
Kyota SAITO
Division of Applied Life Sciences, Graduate School of Agriculture, Kyoto University
Seiichiro MATSUMOTO
Tokyo Laboratory, Yuki Gosei Kogyo Co., Ltd.
Mie SASAKI
Tokyo Laboratory, Yuki Gosei Kogyo Co., Ltd.
Yoichi MIKAMI
Tokyo Laboratory, Yuki Gosei Kogyo Co., Ltd.
Sakayu SHIMIZU
Division of Applied Life Sciences, Graduate School of Agriculture, Kyoto University

Keywords: 2-deoxyribose 5-phosphate, 2-deoxyribose, deoxyriboaldolase, alcoholic fermentation, 2′-deoxyribonucleoside

JOURNAL FREE ACCESS

2006 Volume 70 Issue 6 Pages 1371-1378

DOI https://doi.org/10.1271/bbb.50648

Details

Abstract

2-Deoxyribose 5-phosphate production through coupling of the alcoholic fermentation system of baker’s yeast and deoxyriboaldolase-expressing Escherichia coli was investigated. In this process, baker’s yeast generates fructose 1,6-diphosphate from glucose and inorganic phosphate, and then the E. coli convert the fructose 1,6-diphosphate into 2-deoxyribose 5-phosphate via D-glyceraldehyde 3-phosphate. Under the optimized conditions with toluene-treated yeast cells, 356 mM (121 g/l) fructose 1,6-diphosphate was produced from 1,111 mM glucose and 750 mM potassium phosphate buffer (pH 6.4) with a catalytic amount of AMP, and the reaction supernatant containing the fructose 1,6-diphosphate was used directly as substrate for 2-deoxyribose 5-phosphate production with the E. coli cells. With 178 mM enzymatically prepared fructose 1,6-diphosphate and 400 mM acetaldehyde as substrates, 246 mM (52.6 g/l) 2-deoxyribose 5-phosphate was produced. The molar yield of 2-deoxyribose 5-phosphate as to glucose through the total two step reaction was 22.1%. The 2-deoxyribose 5-phosphate produced was converted to 2-deoxyribose with a molar yield of 85% through endogenous or exogenous phosphatase activity.

Corresponding author

Register with J-STAGE for free!