Human MDR1 and MRP1 Recognize Berberine as Their Transport Substrate

Nobukazu SHITAN; Miyako TANAKA; Koichiro TERAI; Kazumitsu UEDA; Kazufumi YAZAKI

doi:10.1271/bbb.60441

Biochemistry & Molecular Biology Notes

Human MDR1 and MRP1 Recognize Berberine as Their Transport Substrate

Nobukazu SHITAN, Miyako TANAKA, Koichiro TERAI, Kazumitsu UEDA, Kazufumi YAZAKI

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Keywords: ATP-binding cassette (ABC) transporter, berberine, multidrug resistance protein (MDR), multidrug resistance-associated protein (MRP), transport

JOURNAL FREE ACCESS

2007 Volume 71 Issue 1 Pages 242-245

DOI https://doi.org/10.1271/bbb.60441

View "Advance Publication" version (January 7, 2007).

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Abstract

To examine whether human ATP-binding cassette (ABC) transporters play a role in the detoxification of plant alkaloid berberine, we investigated berberine transport using multidrug resistance protein1 (MDR1) and multidrug resistance-associated protein1 (MRP1). Cells expressing MDR1 or MRP1 accumulated less berberine. Berberine accumulation depended on the cellular ATP level, and was reversed by typical inhibitors of MDR1, suggesting that human MDR1 and MRP1 directly efflux berberine as their substrate.

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