Bioscience, Biotechnology, and Biochemistry
Online ISSN : 1347-6947
Print ISSN : 0916-8451
Biochemistry & Molecular Biology Notes
A Method for Pmo25-Associated Kinase Assay in Fission Yeast: The Activity Is Dependent on Two GC Kinases Nak1 and Sid1
Kazunori KUMETetsuya GOSHIMAKohji MIYAHARATakashi TODADai HIRATA
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JOURNAL FREE ACCESS

2007 Volume 71 Issue 2 Pages 615-617

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Abstract

In fission yeast, the conserved proteins, MO25/Pmo25, GC kinase/Nak1, Furry/Mor2, NDR kinase/Orb6, and Mob2, constitute the morphogenesis Orb6 network (MOR). Previously we showed that Pmo25 functions as an upstream component of MOR and that it plays a connecting role between the septation initiation network (SIN) and MOR. Here we establish a Pmo25-associated kinase assay and show that the activity is dependent on Nak1/MOR and Sid1/SIN.

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© 2007 by Japan Society for Bioscience, Biotechnology, and Agrochemistry
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