Bioscience, Biotechnology, and Biochemistry
Online ISSN : 1347-6947
Print ISSN : 0916-8451
Biological Chemistry Notes
Molecular Cloning and Expression Patterns of Cu/Zn-Superoxide Dismutases in Developing Soybean Seeds
Masaomi ARAHIRAVan Hai NONGKazunari KADOKURAKeitarou KIMURAKyoko UDAKAChikafusa FUKAZAWA
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JOURNAL FREE ACCESS

1998 Volume 62 Issue 5 Pages 1018-1021

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Abstract

  Assuming that the amount of superoxide radicals generated in vivo correlates with the production of ergastic substances such as storage proteins, the coordinated response of detoxication enzymes such as superoxide dismutases is largely exploited to understand the self-defense systems of plant.
  Here we examined expression of the genes for superoxide dismutases during seed development of soybean. The cDNAs encoding a cytosolic copper/zinc form and an iron form of the above enzyme have been cloned and then employed as probes, separately. Northern blotting results suggested that both superoxide dismutase mRNAs are expressed at the maximum level, preceding a developmental stage when mRNA encoding glycinin, soybean 11S-storage protein, at the maximum.

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© 1998 by Japan Society for Bioscience, Biotechnology, and Agrochemistry
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