Molecular Cloning, Sequencing and Characterization of the Genes for Adenosylcobalamin-dependent Diol Dehydratase of Klebsiella pneumoniae

Takamasa TOBIMATSU; Muneaki AZUMA; Shinji HAYASHI; Kyou-ichi NISHIMOTO; Tetsuo TORAYA

doi:10.1271/bbb.62.1774

Biochemistry & Molecular Biology Notes

Molecular Cloning, Sequencing and Characterization of the Genes for Adenosylcobalamin-dependent Diol Dehydratase of Klebsiella pneumoniae

Takamasa TOBIMATSU, Muneaki AZUMA, Shinji HAYASHI, Kyou-ichi NISHIMOTO, Tetsuo TORAYA

Author information

Keywords: diol dehydratase, adenosylcobalamin, Klebsiella pneumoniae, nucleotide sequence, glycerol dehydratase

JOURNAL FREE ACCESS

1998 Volume 62 Issue 9 Pages 1774-1777

DOI https://doi.org/10.1271/bbb.62.1774

Details

Abstract

Klebsiella pneumoniae and some of the other Enterobacteriaceae form both diol dehydratase and glycerol dehydratase in response to growth substrates. To compare these enzymes produced by the same bacterium, the pdd genes of K. pneumoniae encoding adenosylcobalamin-dependent diol dehydratase were cloned and sequenced. The sequential three open reading frames (pddA, pddB, and pddC genes) encoded polypeptides of 554, 228, and 174 amino acid residues with predicted molecular weights of 60,379(α), 24,401(β), and 19,489(γ), respectively. The deduced amino acid sequences of the subunits were 84-100% and 54-71% identical with those reported for diol dehydratases and glycerol dehydratases, respectively.

Corresponding author

Register with J-STAGE for free!