Identification of Protein Kinase B (PKB) as a Phosphatidylinositol 3,4,5-Trisphosphate Binding Protein in Dictyostelium discoideum

Kenichi TANAKA; Hiroyuki ADACHI; Hiroaki KONISHI; Akihiro IWAMATSU; Katsuya OHKAWA; Toshiyuki SHIRAI; Satoshi NAGATA; Ushio KIKKAWA; Yasuhisa FUKUI

doi:10.1271/bbb.63.368

Biochemistry & Molecular Biology Regular Papers

Identification of Protein Kinase B (PKB) as a Phosphatidylinositol 3,4,5-Trisphosphate Binding Protein in Dictyostelium discoideum

Kenichi TANAKA, Hiroyuki ADACHI, Hiroaki KONISHI, Akihiro IWAMATSU, Katsuya OHKAWA, Toshiyuki SHIRAI, Satoshi NAGATA, Ushio KIKKAWA, Yasuhisa FUKUI

Author information

Kenichi TANAKA
Laboratory of Biological Chemistry, Division of Applied Biological Chemistry, Graduate School of Agriculture and Life Science, University of Tokyo
Hiroyuki ADACHI
Department of Life Sciences, Graduate School of Arts and Sciences, University of Tokyo
Hiroaki KONISHI
Biosignal Research Center, Kobe University
Akihiro IWAMATSU
Central Laboratories for Key Technology, Kirin Brewery Co. Ltd.
Katsuya OHKAWA
Central Laboratories for Key Technology, Kirin Brewery Co. Ltd.
Toshiyuki SHIRAI
Laboratory of Biological Chemistry, Division of Applied Biological Chemistry, Graduate School of Agriculture and Life Science, University of Tokyo
Satoshi NAGATA
Laboratory of Biological Chemistry, Division of Applied Biological Chemistry, Graduate School of Agriculture and Life Science, University of Tokyo
Ushio KIKKAWA
Biosignal Research Center, Kobe University
Yasuhisa FUKUI
Laboratory of Biological Chemistry, Division of Applied Biological Chemistry, Graduate School of Agriculture and Life Science, University of Tokyo

Keywords: phosphatidylinositol 3,4,5-trisphosphate, PI-3 kinase, protein kinase B, Dictyostelium discoideum, phosphatidylinositol 3,4,5-trisphosphate binding protein

JOURNAL FREE ACCESS

1999 Volume 63 Issue 2 Pages 368-372

DOI https://doi.org/10.1271/bbb.63.368

Details

Abstract

We have searched for phosphatidylinositol (PI)-3,4,5-trisphosphate (PIP₃) binding proteins in Dictyostelium discoideum using beads bearing a PIP₃ analogue, PIP₃-APB. One of the binding proteins with a molecular mass of 55 kDa was purified and its amino acid sequence was partially amalyzed. Database searches showed that the analyzed sequence was identical to that of protein kinase B (PKB) of D. discoideum. The specific activity of D. discoideum PKB, when expressed together with constitutively active PI-3 kinase in mammalian cells, was elevated by about three-fold, suggesting that PKB could also act downstream of PI-3 kinase in Dictyostelium cells.

Corresponding author

Register with J-STAGE for free!