Bioscience, Biotechnology, and Biochemistry
Online ISSN : 1347-6947
Print ISSN : 0916-8451
Microbiology & Fermentation Technology Regular Papers
Susceptibility to Hydrogen Peroxide and Catalase Activity of Root Nodule Bacteria
Takuji OHWADAYukika SHIRAKAWAMasayuki KUSUMOTOHiroshi MASUDATetsuya SATO
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1999 Volume 63 Issue 3 Pages 457-462

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Abstract

  The root nodule bacteria (free-living cells) tested had higher susceptibility to hydrogen peroxide (H2O2) than the other genera of aerobic or facultative anaerobic bacteria tested. The catalase activities tended to have a positive correlation with H2O2 resistance among all bacteria tested. Addition of a catalase inhibitor such as 3-amino-1, 2, 4-triazole increased the susceptibility to H2O2. These results suggest that the lower catalase activity brings about the higher susceptibility of root nodule bacteria to H2O2. Root nodule bacteria seemed to have two or three catalase isozymes during growth and their catalase activities were higher in log phase than in stationary phase, contrary to other genera of bacteria tested.

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© 1999 by Japan Society for Bioscience, Biotechnology, and Agrochemistry
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