Aspirin and Salicylic Acid Do not Inhibit Methyl Jasmonate-inducible Expression of a Gene for Ornithine Decarboxylase in Tobacco BY-2 Cells

Shunsuke IMANISHI; Makiko NAKAKITA; Kouji YAMASHITA; Ayami FURUTA; Kaname UTSUNO; Nobuhiko MURAMOTO; Hisae KOJIMA; Kenzo NAKAMURA

doi:10.1271/bbb.64.125

Biochemistry & Molecular Biology Regular Papers

Aspirin and Salicylic Acid Do not Inhibit Methyl Jasmonate-inducible Expression of a Gene for Ornithine Decarboxylase in Tobacco BY-2 Cells

Shunsuke IMANISHI, Makiko NAKAKITA, Kouji YAMASHITA, Ayami FURUTA, Kaname UTSUNO, Nobuhiko MURAMOTO, Hisae KOJIMA, Kenzo NAKAMURA

Author information

Shunsuke IMANISHI
Laboratory of Biochemistry, Department of Biological Mechanisms and Functions, Graduate School of Bioagricultural Science, Nagoya University
Makiko NAKAKITA
Laboratory of Biochemistry, Department of Biological Mechanisms and Functions, Graduate School of Bioagricultural Science, Nagoya University
Kouji YAMASHITA
Laboratory of Biochemistry, Department of Biological Mechanisms and Functions, Graduate School of Bioagricultural Science, Nagoya University
Ayami FURUTA
Laboratory of Biochemistry, Department of Biological Mechanisms and Functions, Graduate School of Bioagricultural Science, Nagoya University
Kaname UTSUNO
Laboratory of Biochemistry, Department of Biological Mechanisms and Functions, Graduate School of Bioagricultural Science, Nagoya University
Nobuhiko MURAMOTO
Laboratory of Biochemistry, Department of Biological Mechanisms and Functions, Graduate School of Bioagricultural Science, Nagoya University
Hisae KOJIMA
Laboratory of Biochemistry, Department of Biological Mechanisms and Functions, Graduate School of Bioagricultural Science, Nagoya University
Kenzo NAKAMURA
Laboratory of Biochemistry, Department of Biological Mechanisms and Functions, Graduate School of Bioagricultural Science, Nagoya University

Keywords: jasmonate induction, salicylic acid, aspirin, ornithine decarboxylase gene of tobacco, tobacco cells

JOURNAL FREE ACCESS

2000 Volume 64 Issue 1 Pages 125-133

DOI https://doi.org/10.1271/bbb.64.125

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Abstract

Similar to the prostanoid-mediated inflammatory response in mammals, jasmonate-mediated wound response in plant leaves is inhibited by salicylic acid (SA) or acetylsalicylate (aspirin). In tobacco BY-2 cells, expression of the gene for ornithine decarboxylase (ODC) involved in putrescine synthesis is rapidly inducible by methyl jasmonate (MeJA). A nuclear gene for ODC isolated from tobacco, gNtODC-1, was an intron-less gene and MeJA induced the expression of a GUS fusion gene with the gNtODC-1 promoter in transformed tobacco cells. Although SA alone did not induce the expression, 0.2 to 20 μM SA increased the MeJA-induced expression of the fusion gene to about two-fold. A similar increase was observed with aspirin but not with 3- or 4-hydroxybenzoic acids. SA at concentrations up to 200 μM did not inhibit the MeJA-induction of mRNAs for the GUS fusion gene and the endogenous gene for ODC.

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