2000 Volume 64 Issue 10 Pages 2259-2261
Guanosine 5′-monophosphate (5′-GMP) and inosine 5′-monophosphate (5′-IMP) are widely used as flavor enhancers. Recently, a novel process for 5′-IMP production by phosphorylation of inosine using guanosine-inosine kinase coupled with ATP regeneration was reported.1) In this study, we demonstrated the practical possibility of producing 5′-GMP by phosphorylation of guanosine using a guanosine-inosine kinase from Exiguobacterium acetylicum coupled with ATP regeneration.
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