Gordonan, an Acidic Polysaccharide with Cell Aggregation-Inducing Activity in Insect BM-N4 Cells, Produced by Gordonia sp.

Tatsuhiko KONDO; Daisuke YAMAMOTO; Akira YOKOTA; Akinori SUZUKI; Hiromichi NAGASAWA; Shohei SAKUDA

doi:10.1271/bbb.64.2388

Organic Chemistry Regular Papers

Gordonan, an Acidic Polysaccharide with Cell Aggregation-Inducing Activity in Insect BM-N4 Cells, Produced by Gordonia sp.

Tatsuhiko KONDO, Daisuke YAMAMOTO, Akira YOKOTA, Akinori SUZUKI, Hiromichi NAGASAWA, Shohei SAKUDA

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Keywords: Gordonia, polysaccharide, cell aggregation, insect cell line

JOURNAL FREE ACCESS

2000 Volume 64 Issue 11 Pages 2388-2394

DOI https://doi.org/10.1271/bbb.64.2388

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Abstract

An acidic polysaccharide, termed gordonan, was isolated from the culture medium of Gordonia sp. as an inducer of cell aggregation in an insect cell line, BM-N4. Gordonan had an average molecular weight of 5×10⁶ and its structure was identified as →3)-4-O-(1-carboxyethyl)-β-D-Manp-(1→4)-β-D-GlcAp-(1→4)-β-D-Glcp-(1→ mainly by acid hydrolysis experiments and NMR analysis. It induces cell aggregation at the concentration of 4 μg/ml. A partially hydrolyzed polysaccharide derived from gordonan with a molecular weight of 5×10⁵ showed weak activity, while any fragment molecules with lower molecular weights prepared from gordonan showed no activity.

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