An Avocado Constituent, Persenone A, Suppresses Expression of Inducible Forms of Nitric Oxide Synthase and Cyclooxygenase in Macrophages, and Hydrogen Peroxide Generation in Mouse Skin

Oe Kyung KIM; Akira MURAKAMI; Daisuke TAKAHASHI; Yoshimasa NAKAMURA; Koji TORIKAI; Ha Won KIM; Hajime OHIGASHI

doi:10.1271/bbb.64.2504

Food & Nutrition Science Notes

An Avocado Constituent, Persenone A, Suppresses Expression of Inducible Forms of Nitric Oxide Synthase and Cyclooxygenase in Macrophages, and Hydrogen Peroxide Generation in Mouse Skin

Oe Kyung KIM, Akira MURAKAMI, Daisuke TAKAHASHI, Yoshimasa NAKAMURA, Koji TORIKAI, Ha Won KIM, Hajime OHIGASHI

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Keywords: persenone A, inducible nitric oxide synthase, cyclooxygenase-2, hydrogen peroxide

JOURNAL FREE ACCESS

2000 Volume 64 Issue 11 Pages 2504-2507

DOI https://doi.org/10.1271/bbb.64.2504

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Abstract

We investigated the suppressive effects of an avocado constituent, persenone A, on lipopolysaccharide- and interferon-γ-induced inducible nitric oxide synthase (iNOS) and cyclooxygenase (COX-2) in a mouse macrophage cell line RAW 264.7. Persenone A at concentration of 20 μM almost completely suppressed both iNOS and COX-2 protein expression. In mouse skin, double treatments with persenone A (810 nmol) significantly suppressed double 12-O-tetradecanoylphorbol-13-acetate (TPA, 8.1 nmol) application-induced hydrogen peroxide (H₂O₂) generation. Treatment with persenone A before the second TPA treatment was sufficient to inhibit H₂O₂ generation, while the first treatment was not. This study thus suggests that persenone A is a possible agent to prevent inflammation-associated diseases including cancer.

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