Bioscience, Biotechnology, and Biochemistry
Online ISSN : 1347-6947
Print ISSN : 0916-8451
Microbiology & Fermentation Technology
Characterization of Nucleoside Diphosphate Kinase from Moderately Halophilic Eubacteria
Yasushi YONEZAWAHiroko TOKUNAGAMatsujiro ISHIBASHIMasao TOKUNAGA
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JOURNAL FREE ACCESS

2001 Volume 65 Issue 10 Pages 2343-2346

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Abstract

Nucleoside diphosphate kinase was purified to apparent homogeneity from naturally isolated moderately halophilic eubacteria by ATP-agarose and phenyl-5PW column chromatographies. The molecular mass of this enzyme was 15 kDa by time-of-flight mass-spectrometry. This protein showed anomalous mobility on SDS-PAGE which is typical of a halophilic protein. It was stable and active over a wide range of salt concentrations, from 0 to 4.0 M NaCl.

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© 2001 by Japan Society for Bioscience, Biotechnology, and Agrochemistry
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