Isolation and Primary Structural Analysis of Two Conjugated Polyketone Reductases from Candida parapsilosis

Aklani-Rose G.D. HIDALGO; Muhammad Ali AKOND; Keiko KITA; Michihiko KATAOKA; Sakayu SHIMIZU

doi:10.1271/bbb.65.2785

Biochemistry & Molecular Biology

Isolation and Primary Structural Analysis of Two Conjugated Polyketone Reductases from Candida parapsilosis

Aklani-Rose G.D. HIDALGO, Muhammad Ali AKOND, Keiko KITA, Michihiko KATAOKA, Sakayu SHIMIZU

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Keywords: conjugated polyketone reductase, aldoketo reductase, asymmetric reduction, Candida parapsilosis, optically active alcohols

JOURNAL FREE ACCESS

2001 Volume 65 Issue 12 Pages 2785-2788

DOI https://doi.org/10.1271/bbb.65.2785

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Abstract

Two conjugated polyketone reductases (CPRs) were isolated from Candida parapsilosis IFO 0708. The primary structures of CPRs (C1 and C2) were analyzed by amino acid sequencing. The amino acid sequences of both enzymes had high similarity to those of several proteins of the aldo-keto-reductase (AKR) superfamily. However, several amino acid residues in the putative active sites of AKRs were not conserved in CPRs-C1 and -C2.

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