Bioscience, Biotechnology, and Biochemistry
Online ISSN : 1347-6947
Print ISSN : 0916-8451
Food & Nutrition Science
Purification of Soluble β-Glucan with Immune-enhancing Activity from the Cell Wall of Yeast
Jung-Nam LEEDo-Youn LEEIn-Hye JIGi-Eun KIMHye Nam KIMJeongwon SOHNSangduk KIMChan-Wha KIM
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2001 Volume 65 Issue 4 Pages 837-841

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Abstract

β-Glucan, one of the major cell wall components of Saccharomyces cerevisiae, has been found to enhance immune functions, especially by activating macrophages. However, a major obstacle to the clinical application of β-(1→3)-glucan is its low solubility in aqueous media. In this study, soluble β-glucan, free of mannoprotein, was prepared, and its effects on TNF-α secretion and phagocytosis by macrophages were evaluated. β-Glucan was first rendered soluble from the yeast cell wall by alkaline extraction (glucan-p1). The extract contained 2.8% of protein which was subsequently removed by successive DEAE-cellulose and ConA chromatography. β-Glucan thus prepared was completely free of mannoprotein and was soluble at neutral pH (glucan-p3). The effects of β-glucan on phagocytosis and TNF-α release activity were investigated. While glucan-p1 moderately induced TNF-α secretion at 200 μg/ml (550 pg of TNF-α/5×105 cells), glucan-p3 markedly stimulated macrophages at 200 μg/ml (2,860 pg of TNF-α/5×105 cells). Furthermore, glucan-p3 stimulated phagocytosis about 20% more than glucan-p1 did. In conclusion, we purified watersoluble β-glucan which was completely devoid of mannoprotein and effectively stimulated the macrophage function, enabling it to be used as an intravenous injection for sepsis.

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© 2001 by Japan Society for Bioscience, Biotechnology, and Agrochemistry
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