Anti-tumor Promoting Activity of Bufadienolides from Kalanchoe pinnata and K. daigremontiana × butiflora

Unang SUPRATMAN; Tomoyuki FUJITA; Kohki AKIYAMA; Hideo HAYASHI; Akira MURAKAMI; Hirofumi SAKAI; Koichi KOSHIMIZU; Hajime OHIGASHI

doi:10.1271/bbb.65.947

Food & Nutrition Science

Anti-tumor Promoting Activity of Bufadienolides from Kalanchoe pinnata and K. daigremontiana × butiflora

Unang SUPRATMAN, Tomoyuki FUJITA, Kohki AKIYAMA, Hideo HAYASHI, Akira MURAKAMI, Hirofumi SAKAI, Koichi KOSHIMIZU, Hajime OHIGASHI

Author information

Unang SUPRATMAN
Division of Applied Biological Chemistry, Graduate School of Agriculture and Life Sciences, Osaka Prefecture University
Tomoyuki FUJITA
Division of Applied Biological Chemistry, Graduate School of Agriculture and Life Sciences, Osaka Prefecture University
Kohki AKIYAMA
Division of Applied Biological Chemistry, Graduate School of Agriculture and Life Sciences, Osaka Prefecture University
Hideo HAYASHI
Division of Applied Biological Chemistry, Graduate School of Agriculture and Life Sciences, Osaka Prefecture University
Akira MURAKAMI
Department of Biotechnological Science, Faculty of Biology-oriented Science and Technology, Kinki University
Hirofumi SAKAI
Department of Biotechnological Science, Faculty of Biology-oriented Science and Technology, Kinki University
Koichi KOSHIMIZU
Department of Biotechnological Science, Faculty of Biology-oriented Science and Technology, Kinki University
Hajime OHIGASHI
Division of Applied Life Sciences, Graduate School of Agriculture, Kyoto University

Keywords: bufadienolide, Epstein-Barr virus, anti-tumor promoter, Kalanchoe pinnata, Kalanchoe daigremontiana×tubiflora

JOURNAL FREE ACCESS

2001 Volume 65 Issue 4 Pages 947-949

DOI https://doi.org/10.1271/bbb.65.947

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Abstract

Five bufadienolides (1-5) isolated from the leaves of Kalanchoe pinnata and K. daigremontiana×tubiflora (Crassulaceae) were examined for their inhibitory effects on Epstein-Barr virus early antigen (EBV-EA) activation in Raji cells induced by the tumor promoter, 12-O-tetradecanoylphorbol-13-acetate. All bufadienolides showed inhibitory activity, and bryophyllin A (1) exhibited the most marked inhibition (IC₅₀=0.4 μM) among the tested compounds. Bryophyllin C (2), a reduction analogue of 1, and bersaldegenin-3-acetate (3) lacking the orthoacetate moiety were less active. These results strongly suggest that bufadienolides are potential cancer chemopreventive agents.

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