Cloned Cytosine Deaminase Gene Expression of Bifidobacterium longum and Application to Enzyme/Pro-drug Therapy of Hypoxic Solid Tumors

Toshiyuki NAKAMURA; Takayuki SASAKI; Minoru FUJIMORI; Kazuyuki YAZAWA; Yasunobu KANO; Jun AMANO; Shun'ichiro TANIGUCHI

doi:10.1271/bbb.66.2362

Biochemistry & Molecular Biology Regular Papers

Cloned Cytosine Deaminase Gene Expression of Bifidobacterium longum and Application to Enzyme/Pro-drug Therapy of Hypoxic Solid Tumors

Toshiyuki NAKAMURA, Takayuki SASAKI, Minoru FUJIMORI, Kazuyuki YAZAWA, Yasunobu KANO, Jun AMANO, Shun'ichiro TANIGUCHI

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Keywords: Bifidobacterium longum, cytosine deaminase, enzyme/pro-drug therapy, pBLES100- S-eCD

JOURNAL FREE ACCESS

2002 Volume 66 Issue 11 Pages 2362-2366

DOI https://doi.org/10.1271/bbb.66.2362

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Abstract

Bifidobacterium longum is a nonpathogenic anaerobic bacterium among normal bacterial flora. Recently, it was reported that B. longum accumulated in hypoxic solid tumors. The gene of interest was expressed in transfected B. longum by the shuttle vector pBLES100 in solid tumors. In this report, we constructed pBLES100-S-eCD, which included the cytosine deaminase gene. We confirmed by western blotting that transfected B. longum produced cytosine deaminase. In addition, transfected B. longum produced cytosine deaminase that converted 5-fluorocytosine into 5-fluorouracil. B. longum could be useful for enzyme/pro-drug therapy of hypoxic solid tumors.

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