OsRALyase1, a Putative F-Box Protein Identified in Rice, Oryza sativa, with Enzyme Activity Identical to That of Wheat RALyase

Yasuhiro ITO; Akihiko OZAWA; Tatsuya SAWASAKI; Yaeta ENDO; Kozo OCHI; Yuzuru TOZAWA

doi:10.1271/bbb.66.2727

Biochemistry & Molecular Biology Notes

OsRALyase1, a Putative F-Box Protein Identified in Rice, Oryza sativa, with Enzyme Activity Identical to That of Wheat RALyase

Yasuhiro ITO, Akihiko OZAWA, Tatsuya SAWASAKI, Yaeta ENDO, Kozo OCHI, Yuzuru TOZAWA

Author information

Keywords: rice (Oryza sativa), RALyase, sarcin-ricin (S/R) domain, F-box, wheat-germ protein expression system

JOURNAL FREE ACCESS

2002 Volume 66 Issue 12 Pages 2727-2731

DOI https://doi.org/10.1271/bbb.66.2727

Details

Abstract

A rice gene, OsRALyase1, encoding a product similar to wheat ribosomal RNA apurinic site specific lyase (RALyase), was isolated and expressed in vitro. An open reading frame of the gene predicted a protein of 476 amino acid residues with 75% identity to RALyase and contained an F-box-like motif in its amino terminal region. The rice gene product expressed in a wheat-germ protein expression system had the same characteristics as its wheat counterpart, cleaving a specific depurinated site of the 28S rRNA sarcin-ricin domain.

Corresponding author

Register with J-STAGE for free!