Purification of Conjugated Linoleic Acid Isomers through a Process Including Lipase-catalyzed Selective Esterification

Toshihiro NAGAO; Yoshie YAMAUCHI-SATO; Akio SUGIHARA; Toshio IWATA; Koji NAGAO; Teruyoshi YANAGITA; Shuji ADACHI; Yuji SHIMADA

doi:10.1271/bbb.67.1429

Biochemistry & Molecular Biology Notes

Purification of Conjugated Linoleic Acid Isomers through a Process Including Lipase-catalyzed Selective Esterification

Toshihiro NAGAO, Yoshie YAMAUCHI-SATO, Akio SUGIHARA, Toshio IWATA, Koji NAGAO, Teruyoshi YANAGITA, Shuji ADACHI, Yuji SHIMADA

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Keywords: conjugated linoleic acid, Candida rugosa lipase, esterification, urea adduct fractionation, molecular distillation

JOURNAL FREE ACCESS

2003 Volume 67 Issue 6 Pages 1429-1433

DOI https://doi.org/10.1271/bbb.67.1429

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Abstract

A mixture of conjugated linoleic acids (CLAs) was prepared by alkali conjugation of high purity linoleic acid. The preparation contained 45.1 wt% cis-9, trans-11 (c9,t11)-CLA, 46.8 wt% trans-10, cis-12 (t10,c12)-CLA, and 5.3 wt% other CLAs. A process comprising Candida rugosa lipase-catalyzed selective esterification with lauryl alcohol, molecular distillation, and urea adduct fractionation under strict conditions in ethanol was very effective for purification of c9,t11- and t10,c12-CLAs. In particular, the urea adduct fractionation efficiently eliminated CLAs except c9,t11- and t10,c12-isomers. Purification of c9,t11- and t10,c12-CLAs from 1.0 kg of the CLA mixture increased the c9,t11-CLA purity to 93.1% with 34% recovery of the initial content, and increased the t10,c12-CLA purity to 95.3% with 31% recovery.

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