Identification of the Lantibiotic Nisin Q, a New Natural Nisin Variant Produced by Lactococcus lactis 61-14 Isolated from a River in Japan

Takeshi ZENDO; Masanori FUKAO; Kyoko UEDA; Tomoko HIGUCHI; Jiro NAKAYAMA; Kenji SONOMOTO

doi:10.1271/bbb.67.1616

Microbiology & Fermentation Technology Communication

Identification of the Lantibiotic Nisin Q, a New Natural Nisin Variant Produced by Lactococcus lactis 61-14 Isolated from a River in Japan

Takeshi ZENDO, Masanori FUKAO, Kyoko UEDA, Tomoko HIGUCHI, Jiro NAKAYAMA, Kenji SONOMOTO

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Keywords: nisin, bacteriocin, lantibiotic, lactic acid bacteria, Lactococcus lactis

JOURNAL FREE ACCESS

2003 Volume 67 Issue 7 Pages 1616-1619

DOI https://doi.org/10.1271/bbb.67.1616

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Abstract

Lactococcus lactis 61-14 isolated from river water produced a bacteriocin active against a wide range of Gram-positive bacteria. N-terminal amino acid sequencing, mass spectral analysis of the purified bacteriocin, and genetic analysis using nisin-specific primers showed that the bacteriocin was a new natural nisin variant, termed nisin Q. Nisin Q and nisin A differ in four amino acids in the mature peptide and two in the leader sequence.

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