MpFAE3, a β-Ketoacyl-CoA Synthase Gene in the Liverwort Marchantia polymorpha L., Is Preferentially Involved in Elongation of Palmitic Acid to Stearic Acid

Masataka KAJIKAWA; Katsuyuki T. YAMATO; Hiroyuki KANAMARU; Eiji SAKURADANI; Sakayu SHIMIZU; Hideya FUKUZAWA; Yasuyoshi SAKAI; Kanji OHYAMA

doi:10.1271/bbb.67.1667

Biochemistry & Molecular Biology Regular Papers

MpFAE3, a β-Ketoacyl-CoA Synthase Gene in the Liverwort Marchantia polymorpha L., Is Preferentially Involved in Elongation of Palmitic Acid to Stearic Acid

Masataka KAJIKAWA, Katsuyuki T. YAMATO, Hiroyuki KANAMARU, Eiji SAKURADANI, Sakayu SHIMIZU, Hideya FUKUZAWA, Yasuyoshi SAKAI, Kanji OHYAMA

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Masataka KAJIKAWA
Laboratory of Plant Molecular Biology, Division of Integrated Life Science, Graduate School of Biostudies, Kyoto University
Katsuyuki T. YAMATO
Laboratory of Plant Molecular Biology, Division of Integrated Life Science, Graduate School of Biostudies, Kyoto University
Hiroyuki KANAMARU
Laboratory of Fermentation Physiology and Applied Microbiology, Division of Applied Life Sciences, Graduate School of Agriculture, Kyoto University
Eiji SAKURADANI
Laboratory of Fermentation Physiology and Applied Microbiology, Division of Applied Life Sciences, Graduate School of Agriculture, Kyoto University
Sakayu SHIMIZU
Laboratory of Fermentation Physiology and Applied Microbiology, Division of Applied Life Sciences, Graduate School of Agriculture, Kyoto University
Hideya FUKUZAWA
Laboratory of Plant Molecular Biology, Division of Integrated Life Science, Graduate School of Biostudies, Kyoto University
Yasuyoshi SAKAI
Laboratory of Microbial Biotechnology, Division of Applied Life Sciences, Graduate School of Agriculture, Kyoto University
Kanji OHYAMA
Laboratory of Plant Molecular Biology, Division of Integrated Life Science, Graduate School of Biostudies, Kyoto University
Current address: Laboratory of Plant Molecular Genetics, Research Institute of Agricultural Resources, Ishikawa Agricultural College

Keywords: fatty acid chain elongation, heterologous expression, overexpression, bryophyte, β-ketoacyl-CoA synthase (KCS)

JOURNAL FREE ACCESS

2003 Volume 67 Issue 8 Pages 1667-1674

DOI https://doi.org/10.1271/bbb.67.1667

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Abstract

Fatty acid chain elongation is a crucial step in the biosynthesis of long chain fatty acids. An essential reaction in the elongation process is condensation of malonyl-CoA with acyl-CoA, which is catalyzed by β-ketoacyl-CoA synthase (KCS) in plants. We have isolated and characterized the MpFAE3 gene, one of the KCS gene family in the liverwort Marchantia polymorpha. Transgenic M. polymorpha plants overexpressing MpFAE3 accumulate fatty acids 18:0, 20:0, and 22:0. In these plants, the amount of 16:0 is reduced to 50% of wild type. In a heterologous assay, transgenic methylotrophic yeast expressing the MpFAE3 gene accumulates fatty acid 18:0 and generates several longer fatty acids which are not detectable in the control, accompanied by a decrease of 16:0. These observations indicate that the MpFAE3 protein is preferentially involved in the elongation of 16:0 to 18:0 and also in the subsequent steps of 18:0 to 20:0 and 20:0 to 22:0 in M. polymorpha.

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