rDNA ITS Sequence of Rhizopus oryzae: Its Application to Classification and Identification of Lactic Acid Producers

Ayumi ABE; Teruo SONE; I Nengah SUJAYA; Katsuichi SAITO; Yuji ODA; Kozo ASANO; Fusao TOMITA

doi:10.1271/bbb.67.1725

Microbiology & Fermentation Technology Regular Papers

rDNA ITS Sequence of Rhizopus oryzae: Its Application to Classification and Identification of Lactic Acid Producers

Ayumi ABE, Teruo SONE, I Nengah SUJAYA, Katsuichi SAITO, Yuji ODA, Kozo ASANO, Fusao TOMITA

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Keywords: Rhizopus oryzae, ribosomal RNA-encoding DNA (rDNA), internal transcribed spacer (ITS), selective polymerase chain reaction (PCR), lactic acid fermentation

JOURNAL FREE ACCESS

2003 Volume 67 Issue 8 Pages 1725-1731

DOI https://doi.org/10.1271/bbb.67.1725

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Abstract

Rhizopus oryzae is an important organism for its production of organic acids such as lactic acid, fumaric acid, etc. To date, there were no easy methods to classify strains according to their acid production. The sequences of the ribosomal RNA-encoding DNA (rDNA) internal transcribed spacer (ITS) region of 64 strains of R. oryzae were analyzed and found to conserve mutations correspond to acid production. We have devised a way to use these mutations for a novel method to identify lactic-acid-producing Rhizopus oryzae, by designing specific polymerase chain reaction (PCR) primers on them. Touch down PCR using these primers amplified the ITS DNA of lactic acid producers specifically. By this method, we could isolate lactic acid producing strains from Indonesian fermented foods.

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