Isolation and Characterization of Phenol-catabolizing Bacteria from a Coking Plant

Wael S. El-SAYED; Mohamed K. IBRAHIM; Mohamed Abu-SHADY; Fawkia El-BEIH; Naoya OHMURA; Hiroshi SAIKI; Akikazu ANDO

doi:10.1271/bbb.67.2026

Microbiology & Fermentation Technology Notes

Isolation and Characterization of Phenol-catabolizing Bacteria from a Coking Plant

Wael S. El-SAYED, Mohamed K. IBRAHIM, Mohamed Abu-SHADY, Fawkia El-BEIH, Naoya OHMURA, Hiroshi SAIKI, Akikazu ANDO

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Keywords: phenol, degradation, tolerance, meta-pathway

JOURNAL FREE ACCESS

2003 Volume 67 Issue 9 Pages 2026-2029

DOI https://doi.org/10.1271/bbb.67.2026

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Abstract

New phenol degrading bacteria with high biodegradation activity and high tolerance were isolated as Burkholderia cepacia PW3 and Pseudomonas aeruginosa AT2. Both isolates could grow aerobically on phenol as a sole carbon source even at 3 g/l. The whole-cell kinetic properties for phenol degradation by strains PW3 and AT2 showed a V_max of 0.321 and 0.253 mg/l/min/(mg protein), respectively. The metabolic pathways for phenol biodegradation in both strains were assigned to the meta-cleavage activity of catechol 2,3-dioxygenase.

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