Microbial Production of 2-Deoxyribose 5-Phosphate from Acetaldehyde and Triosephosphate for the Synthesis of 2′-Deoxyribonucleosides

Jun OGAWA; Kyota SAITO; Takafumi SAKAI; Nobuyuki HORINOUCHI; Takako KAWANO; Seiichiro MATSUMOTO; Mie SASAKI; Yoichi MIKAMI; Sakayu SHIMIZU

doi:10.1271/bbb.67.933

Microbiology & Fermentation Technology Preliminary Communication

Microbial Production of 2-Deoxyribose 5-Phosphate from Acetaldehyde and Triosephosphate for the Synthesis of 2′-Deoxyribonucleosides

Jun OGAWA, Kyota SAITO, Takafumi SAKAI, Nobuyuki HORINOUCHI, Takako KAWANO, Seiichiro MATSUMOTO, Mie SASAKI, Yoichi MIKAMI, Sakayu SHIMIZU

Author information

Jun OGAWA
Division of Applied Life Sciences, Graduate School of Agriculture, Kyoto University
Kyota SAITO
Division of Applied Life Sciences, Graduate School of Agriculture, Kyoto University
Takafumi SAKAI
Division of Applied Life Sciences, Graduate School of Agriculture, Kyoto University
Nobuyuki HORINOUCHI
Division of Applied Life Sciences, Graduate School of Agriculture, Kyoto University
Takako KAWANO
Division of Applied Life Sciences, Graduate School of Agriculture, Kyoto University
Seiichiro MATSUMOTO
Tokyo Laboratory, Yuki Gosei Kogyo Co., Ltd.
Mie SASAKI
Tokyo Laboratory, Yuki Gosei Kogyo Co., Ltd.
Yoichi MIKAMI
Tokyo Laboratory, Yuki Gosei Kogyo Co., Ltd.
Sakayu SHIMIZU
Division of Applied Life Sciences, Graduate School of Agriculture, Kyoto University

Keywords: 2-deoxyriobse 5-phosphate, 2′-deoxyribonucleoside, deoxyriboaldolase, triosephosphate, Klebsiella pneumoniae

JOURNAL FREE ACCESS

2003 Volume 67 Issue 4 Pages 933-936

DOI https://doi.org/10.1271/bbb.67.933

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Abstract

2-Deoxyribose 5-phosphate was produced from acetaldehyde and dihydroxyacetone phosphate via D-glyceraldehyde 3-phosphate by Klebsiella pneumoniae B-4-4 through deoxyriboaldolase- and triosephosphate isomerase-catalyzing reactions. Under the optimum conditions, 98.7 mM 2-deoxyribose 5-phosphate was produced from 200 mM acetaldehyde and 117 mM dihydroxyacetone phosphate in 2 h with a molar yield of 84%. The 2-deoxyriobse 5-phosphate produced was directly transformed to 2′-deoxyribonucleoside by phosphopentomutase- and nucleoside phosphorylase-catalyzing reactions.

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