Comparative Characterization of the oah2 Gene Homologous to the oah1 of Thermus thermophilus HB8

Tomonori IWAMA; Hideo HOSOKAWA; Weimin LIN; Hideaki SHIMIZU; Keiichi KAWAI; Shuzo YAMAGATA

doi:10.1271/bbb.68.1357

Microbiology & Fermentation Technology Notes

Comparative Characterization of the oah2 Gene Homologous to the oah1 of Thermus thermophilus HB8

Tomonori IWAMA, Hideo HOSOKAWA, Weimin LIN, Hideaki SHIMIZU, Keiichi KAWAI, Shuzo YAMAGATA

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Keywords: O-acetylhomoserine sulfhydrylase, Thermus thermophilus HB8, oah1, oah2

JOURNAL FREE ACCESS

2004 Volume 68 Issue 6 Pages 1357-1361

DOI https://doi.org/10.1271/bbb.68.1357

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Abstract

The oah2 gene homologous to the oah1 of Thermus thermophilus HB8 was cloned and sequenced. It comprised 1,236 bp encoding a protein of 412 amino acid residues and was overexpressed. The gene product, also having O-acetyl-L-homoserine sulfhydrylase (EC 4.2.99.10) activity, was purified to homogeneity and characterized comparatively with the oah1 product. The two proteins shared many characteristics.

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