Expression and Purification of the Bacillus subtilis Thioredoxin Superfamily Protein YkvV

Ryoichi TANAKA; Yoko ARAKI; Makoto MIZUKAMI; Akira MIYAUCHI; Matsujiro ISHIBASHI; Hiroko TOKUNAGA; Masao TOKUNAGA

doi:10.1271/bbb.68.1801

Microbiology & Fermentation Technology Note

Expression and Purification of the Bacillus subtilis Thioredoxin Superfamily Protein YkvV

Ryoichi TANAKA, Yoko ARAKI, Makoto MIZUKAMI, Akira MIYAUCHI, Matsujiro ISHIBASHI, Hiroko TOKUNAGA, Masao TOKUNAGA

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Keywords: Brevibacillus choshinensis, Bacillus subtilis, ykvV, thiol-disulfide oxidoreductase, secretion

JOURNAL FREE ACCESS

2004 Volume 68 Issue 8 Pages 1801-1804

DOI https://doi.org/10.1271/bbb.68.1801

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Abstract

Bacillus subtilis YkvV protein, an extracellular thioredoxin superfamily protein, was successfully expressed both in Brevibacillus choshinensis culture medium using an efficient promoter and the secretion signal of its surface layer protein, and in Escherichia coli cytoplasm with the amino-terminal His-tag (His-YkvV). His-YkvV was purified to homogeneity by Ni-NTA column. Both secreted YkvV and purified His-YkvV exhibited thiol-disulfide oxidoreductase activity.

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