Cloning and Expression Analysis of a MAPKKK Gene and a Novel Nodulin Gene of Lotus japonicus

Natsuko KINOSHITA; Yasuhiro OOKI; Yuichi DEGUCHI; Svetlana A. CHECHETKA; Hiroshi KOUCHI; Yosuke UMEHARA; Katsura IZUI; Shingo HATA

doi:10.1271/bbb.68.1805

Biochemistry & Molecular Biology Notes

Cloning and Expression Analysis of a MAPKKK Gene and a Novel Nodulin Gene of Lotus japonicus

Natsuko KINOSHITA, Yasuhiro OOKI, Yuichi DEGUCHI, Svetlana A. CHECHETKA, Hiroshi KOUCHI, Yosuke UMEHARA, Katsura IZUI, Shingo HATA

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Keywords: legume, mitogen-activated protein kinase cascade, nitrogen fixation, phylogenetic tree, root nodule

JOURNAL FREE ACCESS

2004 Volume 68 Issue 8 Pages 1805-1807

DOI https://doi.org/10.1271/bbb.68.1805

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Abstract

We isolated a cDNA encoding mitogen-activated protein kinase kinase kinase alpha, designated LjM3Kalpha, from Lotus japonicus, a model legume. The gene was expressed constitutively in roots, root nodules, and shoots. We also identified a novel nodulin gene, LjNUF, that shows specific expression in nodules. LjNUF resembles the C-terminal half of a hypothetical protein (pir//D85436), the N-terminal half of which is similar to a portion of mitogen-activated protein kinase kinase kinase gamma. Although LjNUF was predicted to be a secreted protein, its function remains to be clarified.

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