IL-10 Augments Antibody Production in in Vitro Immunized Lymphocytes by Inducing a Th2-Type Response and B Cell Maturation

Qianghua XU; Yoshinori KATAKURA; Makiko YAMASHITA; Shengguo FANG; Takashi TAMURA; Shin-ei MATSUMOTO; Yoshihiro AIBA; Kiichiro TERUYA; Kazuhiro OSADA; Ryuhei NISHIKAWA; Sanetaka SHIRAHATA

doi:10.1271/bbb.68.2279

Biochemistry & Molecular Biology Regular Papers

IL-10 Augments Antibody Production in in Vitro Immunized Lymphocytes by Inducing a Th2-Type Response and B Cell Maturation

Qianghua XU, Yoshinori KATAKURA, Makiko YAMASHITA, Shengguo FANG, Takashi TAMURA, Shin-ei MATSUMOTO, Yoshihiro AIBA, Kiichiro TERUYA, Kazuhiro OSADA, Ryuhei NISHIKAWA, Sanetaka SHIRAHATA

Author information

Qianghua XU
Department of Genetic Resources Technology, Faculty of Agriculture, Kyushu University
College of Life Sciences, Zhejiang University
Key Laboratory of Conservation Genetics and Reproductive Biology for Endangered Wild Animals, Ministry of Education
Yoshinori KATAKURA
Department of Genetic Resources Technology, Faculty of Agriculture, Kyushu University
Makiko YAMASHITA
Department of Genetic Resources Technology, Faculty of Agriculture, Kyushu University
Shengguo FANG
College of Life Sciences, Zhejiang University
Key Laboratory of Conservation Genetics and Reproductive Biology for Endangered Wild Animals, Ministry of Education
Takashi TAMURA
Department of Genetic Resources Technology, Faculty of Agriculture, Kyushu University
Shin-ei MATSUMOTO
Department of Genetic Resources Technology, Faculty of Agriculture, Kyushu University
Yoshihiro AIBA
Department of Genetic Resources Technology, Faculty of Agriculture, Kyushu University
Kiichiro TERUYA
Department of Genetic Resources Technology, Faculty of Agriculture, Kyushu University
Kazuhiro OSADA
Department of Genetic Resources Technology, Faculty of Agriculture, Kyushu University
Ryuhei NISHIKAWA
Department of Genetic Resources Technology, Faculty of Agriculture, Kyushu University
Sanetaka SHIRAHATA
Department of Genetic Resources Technology, Faculty of Agriculture, Kyushu University

Keywords: in vitro immunization, antibody production, IL-10, CD38

JOURNAL FREE ACCESS

2004 Volume 68 Issue 11 Pages 2279-2284

DOI https://doi.org/10.1271/bbb.68.2279

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Abstract

An in vitro immunization (IVI) protocol enables antigen specific antibody production from L-Leucyl-L-Leucine methyl ester (LLME)-treated human peripheral blood lymphocytes (PBL) upon antigen stimulation in the presence of IL-2, IL-4, and muramyl dipeptide. In the course of our studies, we have found that IL-10 added at the antigen sensitization significantly augmented antibody production level from the LLME-treated PBL. In the present study, we tried to demonstrate the role of IL-10 in the augmentation of antibody production in an IVI protocol by clarifying the cytokine expression profiles in CD4⁺ and CD8⁺ T cells. The results showed that IL-10 skewed the Th1/Th2 balance to Th2-type responses by suppressing Th1-type cytokine production and augmenting Th2-type cytokine production in CD4⁺ and CD8⁺ T cells, as well as in CD19⁺ B cells. Furthermore, IL-10 augmented the expression of CD38, an antigen marker of plasma cells, on B cells, which clearly indicates that IL-10 promoted differentiation and maturation of B cells in an IVI protocol. These results indicate that IL-10 plays an important role in setting the cellular milieu to produce antibodies in an IVI protocol.

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